TY - JOUR
T1 - Human Leukocyte Antigens (HLA) Class I and Class II on Sperm Cells Studied at the Serological, Cellular, and Genomic Levels
AU - BISHARA, AMAL
AU - OKSENBERG, JORGE R.
AU - FRANKEL, GADI
AU - MARGALIOTH, EHUD I.J.
AU - PERSITZ, EMANUEL
AU - NELKEN, DAVID
AU - FRIEDMANN, ADAM
AU - BRAUTBAR, CHAIM
PY - 1987/4
Y1 - 1987/4
N2 - ABSTRACT: The expression of human leukocyte antigens (HLA) on highly purified human ejaculated sperm cells was studied using the sensitive enzyme‐linked immunosorbent assay (ELISA) technique and a wide panel of monoclonal antibodies to class I and class II HLA. In addition, the stimulatory capacity of these cells was tested in mixed cultures of lymphocytes and spermatozoa, and the levels of RNA homologous to the HLA class I and class II genes were determined. The results obtained using the ELISA indicate that the class I and class II HLA serologically defined antigens are weakly expressed on the cell surface of the mature spermatozoa. Highly purified sperm cells consistently stimulated heterologous lymphocytes but not when HLA‐DR compatibility was observed between stimulator and responder. The proliferative response of lymphocytes induced by sperm cells was lower than the response obtained in a lymphocyte‐lymphocyte combination, though the kinetics of the response were similar in both cases. In addition, it was found that spermatozoa contained RNA species homologous to HLA class II DRβ and DQβ genes sequences but not to HLA class I sequences. The levels of these RNA species were significantly reduced after interferon stimulation. Lymphocytes that served as positive control were found to contain RNA complementary to both HLA class I and class II genes. 1987 Munksgaard
AB - ABSTRACT: The expression of human leukocyte antigens (HLA) on highly purified human ejaculated sperm cells was studied using the sensitive enzyme‐linked immunosorbent assay (ELISA) technique and a wide panel of monoclonal antibodies to class I and class II HLA. In addition, the stimulatory capacity of these cells was tested in mixed cultures of lymphocytes and spermatozoa, and the levels of RNA homologous to the HLA class I and class II genes were determined. The results obtained using the ELISA indicate that the class I and class II HLA serologically defined antigens are weakly expressed on the cell surface of the mature spermatozoa. Highly purified sperm cells consistently stimulated heterologous lymphocytes but not when HLA‐DR compatibility was observed between stimulator and responder. The proliferative response of lymphocytes induced by sperm cells was lower than the response obtained in a lymphocyte‐lymphocyte combination, though the kinetics of the response were similar in both cases. In addition, it was found that spermatozoa contained RNA species homologous to HLA class II DRβ and DQβ genes sequences but not to HLA class I sequences. The levels of these RNA species were significantly reduced after interferon stimulation. Lymphocytes that served as positive control were found to contain RNA complementary to both HLA class I and class II genes. 1987 Munksgaard
KW - ELISA
KW - Human spermatozoa
KW - MHC genes and products
KW - homologous RNA
KW - mixed lymphocyte culture
KW - γinterferon
UR - http://www.scopus.com/inward/record.url?scp=0023251151&partnerID=8YFLogxK
U2 - 10.1111/j.1600-0897.1987.tb00102.x
DO - 10.1111/j.1600-0897.1987.tb00102.x
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C2 - 2440323
AN - SCOPUS:0023251151
SN - 8755-8920
VL - 13
SP - 97
EP - 103
JO - American Journal of Reproductive Immunology and Microbiology
JF - American Journal of Reproductive Immunology and Microbiology
IS - 4
ER -