Human mast cells express intracellular TRAIL

Beata Berent-Maoz; Souzan Salemi; David Mankuta; Hans Uwe Simon; Francesca Levi-Schaffer

doi:10.1016/j.cellimm.2010.02.003

Human mast cells express intracellular TRAIL

Beata Berent-Maoz, Souzan Salemi, David Mankuta, Hans Uwe Simon, Francesca Levi-Schaffer^*

^*Corresponding author for this work

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Recently we demonstrated that human mast cells (MC) express functional TRAIL death receptors. Here we assessed the expression of TRAIL on both mRNA and protein level in cord blood derived MC (CBMC) and HMC-1. The TRAIL release either spontaneous or induced by LPS, IFN-γ and IgE-dependent activation, was evaluated as well. The protein location was restricted to the intracellular compartment in CBMC, but not in HMC-1. The intracellular TRAIL was not localized inside the granules. The treatment with IFN-γ and LPS up-regulated intracellular TRAIL expression in CBMC, but did not induce its release. These in vitro data show that human MC can produce and express intracellular TRAIL whose location could not be altered by different stimuli.

Original language	English
Pages (from-to)	80-83
Number of pages	4
Journal	Cellular Immunology
Volume	262
Issue number	2
DOIs	https://doi.org/10.1016/j.cellimm.2010.02.003
State	Published - 2010

Bibliographical note

Funding Information:
This work was supported in part by Grant No. 3000002966 from the Chief Scientist’s Office of the Ministry of Health, Israel to F.L.S., a grant from the Aimwell Charitable Trust Foundation (UK) to F.L.S. and by the Swiss National Science Foundation (Grant No. 310000-107526) to H.U.S. B.B.M. received a 2005 EAACI Fellowship Award and GA2LEN Fellowship Award.

Keywords

Apoptosis
Cell activation
Mast cells
TRAIL

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.cellimm.2010.02.003

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title = "Human mast cells express intracellular TRAIL",

abstract = "Recently we demonstrated that human mast cells (MC) express functional TRAIL death receptors. Here we assessed the expression of TRAIL on both mRNA and protein level in cord blood derived MC (CBMC) and HMC-1. The TRAIL release either spontaneous or induced by LPS, IFN-γ and IgE-dependent activation, was evaluated as well. The protein location was restricted to the intracellular compartment in CBMC, but not in HMC-1. The intracellular TRAIL was not localized inside the granules. The treatment with IFN-γ and LPS up-regulated intracellular TRAIL expression in CBMC, but did not induce its release. These in vitro data show that human MC can produce and express intracellular TRAIL whose location could not be altered by different stimuli.",

keywords = "Apoptosis, Cell activation, Mast cells, TRAIL",

author = "Beata Berent-Maoz and Souzan Salemi and David Mankuta and Simon, {Hans Uwe} and Francesca Levi-Schaffer",

note = "Funding Information: This work was supported in part by Grant No. 3000002966 from the Chief Scientist{\textquoteright}s Office of the Ministry of Health, Israel to F.L.S., a grant from the Aimwell Charitable Trust Foundation (UK) to F.L.S. and by the Swiss National Science Foundation (Grant No. 310000-107526) to H.U.S. B.B.M. received a 2005 EAACI Fellowship Award and GA2LEN Fellowship Award.",

year = "2010",

doi = "10.1016/j.cellimm.2010.02.003",

language = "אנגלית",

volume = "262",

pages = "80--83",

journal = "Cellular Immunology",

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AU - Berent-Maoz, Beata

AU - Salemi, Souzan

AU - Mankuta, David

AU - Simon, Hans Uwe

AU - Levi-Schaffer, Francesca

N1 - Funding Information: This work was supported in part by Grant No. 3000002966 from the Chief Scientist’s Office of the Ministry of Health, Israel to F.L.S., a grant from the Aimwell Charitable Trust Foundation (UK) to F.L.S. and by the Swiss National Science Foundation (Grant No. 310000-107526) to H.U.S. B.B.M. received a 2005 EAACI Fellowship Award and GA2LEN Fellowship Award.

PY - 2010

Y1 - 2010

N2 - Recently we demonstrated that human mast cells (MC) express functional TRAIL death receptors. Here we assessed the expression of TRAIL on both mRNA and protein level in cord blood derived MC (CBMC) and HMC-1. The TRAIL release either spontaneous or induced by LPS, IFN-γ and IgE-dependent activation, was evaluated as well. The protein location was restricted to the intracellular compartment in CBMC, but not in HMC-1. The intracellular TRAIL was not localized inside the granules. The treatment with IFN-γ and LPS up-regulated intracellular TRAIL expression in CBMC, but did not induce its release. These in vitro data show that human MC can produce and express intracellular TRAIL whose location could not be altered by different stimuli.

AB - Recently we demonstrated that human mast cells (MC) express functional TRAIL death receptors. Here we assessed the expression of TRAIL on both mRNA and protein level in cord blood derived MC (CBMC) and HMC-1. The TRAIL release either spontaneous or induced by LPS, IFN-γ and IgE-dependent activation, was evaluated as well. The protein location was restricted to the intracellular compartment in CBMC, but not in HMC-1. The intracellular TRAIL was not localized inside the granules. The treatment with IFN-γ and LPS up-regulated intracellular TRAIL expression in CBMC, but did not induce its release. These in vitro data show that human MC can produce and express intracellular TRAIL whose location could not be altered by different stimuli.

KW - Apoptosis

KW - Cell activation

KW - Mast cells

KW - TRAIL

UR - http://www.scopus.com/inward/record.url?scp=77950862195&partnerID=8YFLogxK

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DO - 10.1016/j.cellimm.2010.02.003

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C2 - 20189551

AN - SCOPUS:77950862195

SN - 0008-8749

VL - 262

SP - 80

EP - 83

JO - Cellular Immunology

JF - Cellular Immunology

IS - 2

ER -

Human mast cells express intracellular TRAIL

Abstract

Bibliographical note

Keywords

UN SDGs

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