TY - JOUR
T1 - Human monoclonal autoimmune antibody produced in Vitro
T2 - rheumatoid factor generated by Epstein‐Barr virus‐transformed cell line
AU - Steinitz, Michael
AU - Tamir, Sara
PY - 1982
Y1 - 1982
N2 - The infection of selected lymphocytes from a rheumatoid arthritis patient with Epstein‐Barr virus resulted in an immortalized cell line that secretes a monoclonal rheumatoid factor (RF). The cloned line has been growing for more than 24 months, and constantly produces a monoclonal IgM, λ, 19 S, RF (1‐2 μg/ml/l06 cells). The RF agglutinates human and rabbit IgG (but not IgM) and also protein A‐coated erythrocytes, but fails to do so to mouse, goat and swine IgG‐coated erythrocytes. When bound to immune complexes, this monoclonal RF does not bind complement. In the cell supernatant RF is the only immunoglobulin and it comprises approximately 5% of the total proteins. The affinity of RF to aggregated human IgG, as detected in inhibition experiments, is higher than that of Fe receptors found on human non‐T lymphocytes, K562 and Daudi cell lines.
AB - The infection of selected lymphocytes from a rheumatoid arthritis patient with Epstein‐Barr virus resulted in an immortalized cell line that secretes a monoclonal rheumatoid factor (RF). The cloned line has been growing for more than 24 months, and constantly produces a monoclonal IgM, λ, 19 S, RF (1‐2 μg/ml/l06 cells). The RF agglutinates human and rabbit IgG (but not IgM) and also protein A‐coated erythrocytes, but fails to do so to mouse, goat and swine IgG‐coated erythrocytes. When bound to immune complexes, this monoclonal RF does not bind complement. In the cell supernatant RF is the only immunoglobulin and it comprises approximately 5% of the total proteins. The affinity of RF to aggregated human IgG, as detected in inhibition experiments, is higher than that of Fe receptors found on human non‐T lymphocytes, K562 and Daudi cell lines.
UR - http://www.scopus.com/inward/record.url?scp=0020078033&partnerID=8YFLogxK
U2 - 10.1002/eji.1830120206
DO - 10.1002/eji.1830120206
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C2 - 6281026
AN - SCOPUS:0020078033
SN - 0014-2980
VL - 12
SP - 126
EP - 133
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 2
ER -