Human monoclonal autoimmune antibody produced in Vitro: rheumatoid factor generated by Epstein‐Barr virus‐transformed cell line

Michael Steinitz*, Sara Tamir

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

The infection of selected lymphocytes from a rheumatoid arthritis patient with Epstein‐Barr virus resulted in an immortalized cell line that secretes a monoclonal rheumatoid factor (RF). The cloned line has been growing for more than 24 months, and constantly produces a monoclonal IgM, λ, 19 S, RF (1‐2 μg/ml/l06 cells). The RF agglutinates human and rabbit IgG (but not IgM) and also protein A‐coated erythrocytes, but fails to do so to mouse, goat and swine IgG‐coated erythrocytes. When bound to immune complexes, this monoclonal RF does not bind complement. In the cell supernatant RF is the only immunoglobulin and it comprises approximately 5% of the total proteins. The affinity of RF to aggregated human IgG, as detected in inhibition experiments, is higher than that of Fe receptors found on human non‐T lymphocytes, K562 and Daudi cell lines.

Original languageEnglish
Pages (from-to)126-133
Number of pages8
JournalEuropean Journal of Immunology
Volume12
Issue number2
DOIs
StatePublished - 1982
Externally publishedYes

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