TY - JOUR
T1 - Human oocyte-derived sperm chemoattractant is a hydrophobic molecule associated with a carrier protein
AU - Armon, Leah
AU - Ben-Ami, Ido
AU - Ron-El, Raphael
AU - Eisenbach, Michael
PY - 2014/9
Y1 - 2014/9
N2 - Objective To characterize the nature of the human oocyte-derived chemoattractant. Design Laboratory in vitro study. Setting Academic research institute. Patient(s) Ten healthy sperm donors. Oocyte-conditioned media from women undergoing IVF treatment because of male factor infertility. Intervention(s) Sperm samples were processed by the migration-sedimentation technique. Oocyte-conditioned media were collected 2-3 hours after oocyte stripping. Main Outcome Measure(s) Sperm chemotaxis was assayed in a μ-slide chamber according to the direction of swimming relative to that of the chemical gradient. Result(s) Oocyte-conditioned media treated with proteases did not lose their chemotactic activity; on the contrary, they became more active, with the activity shifted to lower concentrations. When oocyte-conditioned media were subjected to hexane extraction, chemotactic activity was found in both the hydrophobic and aqueous phases. Known mammalian sperm chemoattractants were ruled out as oocyte-derived chemoattractants. Conclusion(s) Our results suggest that the oocyte-derived chemoattractant is a hydrophobic nonpeptide molecule that, in an oocyte-conditioned medium, is associated with a carrier protein that enables its presence in a hydrophilic environment.
AB - Objective To characterize the nature of the human oocyte-derived chemoattractant. Design Laboratory in vitro study. Setting Academic research institute. Patient(s) Ten healthy sperm donors. Oocyte-conditioned media from women undergoing IVF treatment because of male factor infertility. Intervention(s) Sperm samples were processed by the migration-sedimentation technique. Oocyte-conditioned media were collected 2-3 hours after oocyte stripping. Main Outcome Measure(s) Sperm chemotaxis was assayed in a μ-slide chamber according to the direction of swimming relative to that of the chemical gradient. Result(s) Oocyte-conditioned media treated with proteases did not lose their chemotactic activity; on the contrary, they became more active, with the activity shifted to lower concentrations. When oocyte-conditioned media were subjected to hexane extraction, chemotactic activity was found in both the hydrophobic and aqueous phases. Known mammalian sperm chemoattractants were ruled out as oocyte-derived chemoattractants. Conclusion(s) Our results suggest that the oocyte-derived chemoattractant is a hydrophobic nonpeptide molecule that, in an oocyte-conditioned medium, is associated with a carrier protein that enables its presence in a hydrophilic environment.
KW - chemotaxis
KW - oocyte-derived chemoattractant
KW - Sperm chemotaxis
UR - http://www.scopus.com/inward/record.url?scp=84906939949&partnerID=8YFLogxK
U2 - 10.1016/j.fertnstert.2014.06.011
DO - 10.1016/j.fertnstert.2014.06.011
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C2 - 25063727
AN - SCOPUS:84906939949
SN - 0015-0282
VL - 102
SP - 885
EP - 890
JO - Fertility and Sterility
JF - Fertility and Sterility
IS - 3
ER -