Hydrolysis of N'-Benzoyl-Arginine-p-Nitroanilide Stereoisomers as a Phenotypic Test: a Study of Gram-Positive Halotolerant Bacteria

Aharon Oren*, Erwin A. Galinski

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

We tested a variety of halotolerant Gram-positive coccoid bacteria and other halotolerant bacteria for hydrolytic activity towards the L- and the D-isomer of N'-benzoyl-arginine-p-nitroanilide (BAPA). D-BAPA cleaving activity was earlier shown to be associated with endospore-forming bacteria and related organisms. Four groups were distinguished on the basis of their behavior towards the BAPA isomers: 1. D-BAPA cleaving bacteria: several Bacillus strains, two Planococcus species, and Marinococcus hispanicus. 2. Strain M96/12b, an endospore-forming bacterium, that cleaves both isomers. 3. L-BAPA cleaving bacteria: Gram-negative halotolerant bacteria (Halomonas elongata, Vibrio costicola), the Gram-positive coccoid bacterium Micrococcus luteus, and Bacillus pasteurii. 4. A number of Gram-positive cocci that cleave neither L-BAPA nor D-BAPA. Also a few ectoine-producing Bacillus species (B. halophilus, B. pantothenticus) fall in this category. We recommended to include the tests for L-BAPA and D-BAPA hydrolysis in phenotypic tests and numerical taxonomy studies of Gram-positive bacteria.

Original languageAmerican English
Pages (from-to)7-10
Number of pages4
JournalSystematic and Applied Microbiology
Volume17
Issue number1
DOIs
StatePublished - 1994

Bibliographical note

Funding Information:
Acknowledgements.We thank Birgit Amendt for her help in the experimental work. This study was supported by the G.LF., the German-Israeli Foundation for Scientific Research and Development.

Keywords

  • Bacillus
  • D-BAPA
  • DMSO
  • L-BAPA
  • Marinococcus
  • N'-benzoyl-D-arginine-p-nitroanilide
  • N'-benzoyl-D-arginine-p-nitroanilide
  • N'-benzoyl-L-arginine-p-nitroanilide
  • N'-benzoyl-L-arginine-p-nitroanilide
  • Planococcus
  • Salinicoccus
  • dimethylsulfoxide

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