TY - JOUR
T1 - Hydrolysis of N′-benzoyl-d-arginine-p-nitroanilide by members of the Haloanaerobiaceae
T2 - additional evidence that Haloanaerobium praevalens is related to endospore-forming bacteria
AU - Oren, Aharon
AU - Gofshtein-Gandman, Lia V.
AU - Keynan, Alexander
N1 - Funding Information:
This work was supported in part by a grant from the Israeli Ministry of Energy and Infrastructure.
PY - 1989/3
Y1 - 1989/3
N2 - Three out of the four described halophilic obligately anaerobic bacteria of the family Haloanaerobiaceae hydrolyze d-BAPA (N′-benzoyl-d-arginine-p-nitroanilide), while showing no or little l-BAPA hydrolyzing activity. This property was shown earlier to be characteristic only of non-halophilic Gram-positive endospore-forming bacteria of the genera Bacillus and Clostridium. These results suggest that Haloanaerobium praevalens, which has never been shown to produce endospores, but was shown to be related to the endosphere-forming representatives of the Haloanaerobiaceae on the basis of 16S rRNA nucleotide sequence data, shares other properties characteristics of the endospore-forming bacteria. Neither significant d-BAPA nor l-BAPA hydrolyzing activity was found in Sporohalobacter lortetii.
AB - Three out of the four described halophilic obligately anaerobic bacteria of the family Haloanaerobiaceae hydrolyze d-BAPA (N′-benzoyl-d-arginine-p-nitroanilide), while showing no or little l-BAPA hydrolyzing activity. This property was shown earlier to be characteristic only of non-halophilic Gram-positive endospore-forming bacteria of the genera Bacillus and Clostridium. These results suggest that Haloanaerobium praevalens, which has never been shown to produce endospores, but was shown to be related to the endosphere-forming representatives of the Haloanaerobiaceae on the basis of 16S rRNA nucleotide sequence data, shares other properties characteristics of the endospore-forming bacteria. Neither significant d-BAPA nor l-BAPA hydrolyzing activity was found in Sporohalobacter lortetii.
KW - Endospores
KW - Haloanaerobiaceae
KW - Haloanaerobium praevalens
KW - d-BAPA hydrolysis
UR - http://www.scopus.com/inward/record.url?scp=45249129830&partnerID=8YFLogxK
U2 - 10.1111/j.1574-6968.1989.tb03008.x
DO - 10.1111/j.1574-6968.1989.tb03008.x
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AN - SCOPUS:45249129830
SN - 0378-1097
VL - 58
SP - 5
EP - 9
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 1
ER -