TY - JOUR
T1 - Hypochlorous acid activates the heat shock and soxRS systems of Escherichia coli
AU - Dukan, Sam
AU - Dadon, Sara
AU - Smulski, Dana R.
AU - Belkin, Shimshon
PY - 1996/11
Y1 - 1996/11
N2 - A series of plasmids, containing fusions of different stress promoters to lux reporter genes, was used in an attempt to monitor the defense circuits activated upon exposure of Escherichia coli to sublethal doses of free chlorine. A significant level of activation was exhibited by promoters of three heat shuck genes (grpE, dnaK, and lon), in an rpoH-dependent manner. The promoter of micF, a gene under the control of the soxRS regulon, was also strongly induced, but not in a soxR mutant. This induction was not affected by soda and sodB mutations, implying that it did not involve oxygen radical activity. Free-chlorine activation of both heat shuck and soxRS regulons required an exposure of less then 1 s in duration. The oxyR or the SOS regulons were apparently not induced by free chlorine (as judged by lack of activation of katG and recA, respectively), and neither was the universal stress (uspA) protein.
AB - A series of plasmids, containing fusions of different stress promoters to lux reporter genes, was used in an attempt to monitor the defense circuits activated upon exposure of Escherichia coli to sublethal doses of free chlorine. A significant level of activation was exhibited by promoters of three heat shuck genes (grpE, dnaK, and lon), in an rpoH-dependent manner. The promoter of micF, a gene under the control of the soxRS regulon, was also strongly induced, but not in a soxR mutant. This induction was not affected by soda and sodB mutations, implying that it did not involve oxygen radical activity. Free-chlorine activation of both heat shuck and soxRS regulons required an exposure of less then 1 s in duration. The oxyR or the SOS regulons were apparently not induced by free chlorine (as judged by lack of activation of katG and recA, respectively), and neither was the universal stress (uspA) protein.
UR - http://www.scopus.com/inward/record.url?scp=0029827844&partnerID=8YFLogxK
U2 - 10.1128/aem.62.11.4003-4008.1996
DO - 10.1128/aem.62.11.4003-4008.1996
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C2 - 8899987
AN - SCOPUS:0029827844
SN - 0099-2240
VL - 62
SP - 4003
EP - 4008
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
IS - 11
ER -