Identification and characterization of a site-specific tyrosine recombinase within the variable loci of Mycoplasma bovis, Mycoplasma pulmonis and Mycoplasma agalactiae

Yael Ron; Ravenna Flitman-Tene; Kevin Dybvig; David Yogev

doi:10.1016/S0378-1119(02)00679-0

Identification and characterization of a site-specific tyrosine recombinase within the variable loci of Mycoplasma bovis, Mycoplasma pulmonis and Mycoplasma agalactiae

Yael Ron, Ravenna Flitman-Tene, Kevin Dybvig, David Yogev^*

^*Corresponding author for this work

Department of Microbiology and Molecular Genetics

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Three highly mutable loci of the wall-less pathogens Mycoplasma bovis, Mycoplasma pulmonis and Mycoplasma agalactiae undergo high-frequency genomic rearrangements and generate extensive antigenic variation of major surface lipoproteins. Adjacent to each locus, an open reading frame exists as a single chromosomal copy and is predicted to encode a site-specific DNA recombinase exhibiting high homology to the recombinases XerD of Escherichia coli and CodV of Bacillus subtilis. Each of the mycoplasmal proteins are members of the λ integrase family of tyrosine site-specific recombinases and likely mediates site-specific DNA inversions observed within the adjacent, variable loci.

Original language	English
Pages (from-to)	205-211
Number of pages	7
Journal	Gene
Volume	292
Issue number	1-2
DOIs	https://doi.org/10.1016/S0378-1119(02)00679-0
State	Published - 12 Jun 2002

Bibliographical note

Funding Information:
This study was supported in part by the German–Israeli Foundation for Scientific Research and Development (GIF), by research Grant No. IS-2540-95R from BARD, The United States–Israel Binational Agricultural Research and Development Fund by the Israel Academy of Sciences and Humanities Foundation and in part by the US Public Health Service grant AI41113 to K.D. from the National Institutes of Health. Y.R. was supported by the Yeshaya Horowitz Fellowship.

Keywords

Antigenic variation
Lipoprotein
Mycoplasmas
Site-specific DNA inversion
Tyrosine recombinase

Access to Document

10.1016/S0378-1119(02)00679-0

Cite this

@article{1973d68816d84c06af9202e5b76ed63c,

title = "Identification and characterization of a site-specific tyrosine recombinase within the variable loci of Mycoplasma bovis, Mycoplasma pulmonis and Mycoplasma agalactiae",

abstract = "Three highly mutable loci of the wall-less pathogens Mycoplasma bovis, Mycoplasma pulmonis and Mycoplasma agalactiae undergo high-frequency genomic rearrangements and generate extensive antigenic variation of major surface lipoproteins. Adjacent to each locus, an open reading frame exists as a single chromosomal copy and is predicted to encode a site-specific DNA recombinase exhibiting high homology to the recombinases XerD of Escherichia coli and CodV of Bacillus subtilis. Each of the mycoplasmal proteins are members of the λ integrase family of tyrosine site-specific recombinases and likely mediates site-specific DNA inversions observed within the adjacent, variable loci.",

keywords = "Antigenic variation, Lipoprotein, Mycoplasmas, Site-specific DNA inversion, Tyrosine recombinase",

author = "Yael Ron and Ravenna Flitman-Tene and Kevin Dybvig and David Yogev",

note = "Funding Information: This study was supported in part by the German–Israeli Foundation for Scientific Research and Development (GIF), by research Grant No. IS-2540-95R from BARD, The United States–Israel Binational Agricultural Research and Development Fund by the Israel Academy of Sciences and Humanities Foundation and in part by the US Public Health Service grant AI41113 to K.D. from the National Institutes of Health. Y.R. was supported by the Yeshaya Horowitz Fellowship.",

year = "2002",

month = jun,

day = "12",

doi = "10.1016/S0378-1119(02)00679-0",

language = "אנגלית",

volume = "292",

pages = "205--211",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Identification and characterization of a site-specific tyrosine recombinase within the variable loci of Mycoplasma bovis, Mycoplasma pulmonis and Mycoplasma agalactiae

AU - Ron, Yael

AU - Flitman-Tene, Ravenna

AU - Dybvig, Kevin

AU - Yogev, David

N1 - Funding Information: This study was supported in part by the German–Israeli Foundation for Scientific Research and Development (GIF), by research Grant No. IS-2540-95R from BARD, The United States–Israel Binational Agricultural Research and Development Fund by the Israel Academy of Sciences and Humanities Foundation and in part by the US Public Health Service grant AI41113 to K.D. from the National Institutes of Health. Y.R. was supported by the Yeshaya Horowitz Fellowship.

PY - 2002/6/12

Y1 - 2002/6/12

N2 - Three highly mutable loci of the wall-less pathogens Mycoplasma bovis, Mycoplasma pulmonis and Mycoplasma agalactiae undergo high-frequency genomic rearrangements and generate extensive antigenic variation of major surface lipoproteins. Adjacent to each locus, an open reading frame exists as a single chromosomal copy and is predicted to encode a site-specific DNA recombinase exhibiting high homology to the recombinases XerD of Escherichia coli and CodV of Bacillus subtilis. Each of the mycoplasmal proteins are members of the λ integrase family of tyrosine site-specific recombinases and likely mediates site-specific DNA inversions observed within the adjacent, variable loci.

AB - Three highly mutable loci of the wall-less pathogens Mycoplasma bovis, Mycoplasma pulmonis and Mycoplasma agalactiae undergo high-frequency genomic rearrangements and generate extensive antigenic variation of major surface lipoproteins. Adjacent to each locus, an open reading frame exists as a single chromosomal copy and is predicted to encode a site-specific DNA recombinase exhibiting high homology to the recombinases XerD of Escherichia coli and CodV of Bacillus subtilis. Each of the mycoplasmal proteins are members of the λ integrase family of tyrosine site-specific recombinases and likely mediates site-specific DNA inversions observed within the adjacent, variable loci.

KW - Antigenic variation

KW - Lipoprotein

KW - Mycoplasmas

KW - Site-specific DNA inversion

KW - Tyrosine recombinase

UR - http://www.scopus.com/inward/record.url?scp=0037067243&partnerID=8YFLogxK

U2 - 10.1016/S0378-1119(02)00679-0

DO - 10.1016/S0378-1119(02)00679-0

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 12119115

AN - SCOPUS:0037067243

SN - 0378-1119

VL - 292

SP - 205

EP - 211

JO - Gene

JF - Gene

IS - 1-2

ER -

Identification and characterization of a site-specific tyrosine recombinase within the variable loci of Mycoplasma bovis, Mycoplasma pulmonis and Mycoplasma agalactiae

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this