Identification and Characterization of Diploid and Tetraploid in Platycodon grandiflorum

Jeoung Hwa Shin; Yun Gyong Ahn; Ju Hee Jung; Sun Hee Woo; Hag Hyun Kim; Shela Gorinstein; Hee Ock Boo

doi:10.1007/s11130-016-0589-7

Identification and Characterization of Diploid and Tetraploid in Platycodon grandiflorum

Jeoung Hwa Shin, Yun Gyong Ahn, Ju Hee Jung, Sun Hee Woo, Hag Hyun Kim, Shela Gorinstein^*, Hee Ock Boo

^*Corresponding author for this work

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Platycodon grandiflorum (PG), a species of herbaceous flowering perennial plant of the family Campanulaceae, has been used as a traditional oriental medicine for bronchitis, asthma, pulmonary tuberculosis, diabetes, hepatic fibrosis, bone disorders and many others similar diseases and as a food supplement. For the primary profiling of PG gas chromatography coupled with high resolution – time of flight mass spectrometry (GC/HR-TOF MS) was used as an analytical tool. A comparison of optimal extraction of metabolites was carried out with a number of solvents [hexane, methylene chloride, methanol, ethanol, methanol: ethanol (70:30, v:v)]. In extracts with methanol: ethanol (70:30 v:v) were detected higher amounts of metabolites than with other solvents. Principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) plots showed significant differences between the diploid and tetraploid metabolite profiles. Extracts of tetraploid showed higher amounts of amino acids, while extracts of diploid contained more organic acids and sugars. [Figure not available: see fulltext.]

Original language	English
Pages (from-to)	13-19
Number of pages	7
Journal	Plant Foods for Human Nutrition
Volume	72
Issue number	1
DOIs	https://doi.org/10.1007/s11130-016-0589-7
State	Published - 1 Mar 2017

Bibliographical note

Publisher Copyright:
© 2016, Springer Science+Business Media New York.

Keywords

Diploid
Gas chromatography/high resolution time of flight mass spectrometry (GC/HR-TOF MS)
Partial least-squares discriminant analysis (OPLS-DA)
Platycodon grandiflorum (PG)
Tetraploid

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s11130-016-0589-7

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title = "Identification and Characterization of Diploid and Tetraploid in Platycodon grandiflorum",

abstract = "Platycodon grandiflorum (PG), a species of herbaceous flowering perennial plant of the family Campanulaceae, has been used as a traditional oriental medicine for bronchitis, asthma, pulmonary tuberculosis, diabetes, hepatic fibrosis, bone disorders and many others similar diseases and as a food supplement. For the primary profiling of PG gas chromatography coupled with high resolution – time of flight mass spectrometry (GC/HR-TOF MS) was used as an analytical tool. A comparison of optimal extraction of metabolites was carried out with a number of solvents [hexane, methylene chloride, methanol, ethanol, methanol: ethanol (70:30, v:v)]. In extracts with methanol: ethanol (70:30 v:v) were detected higher amounts of metabolites than with other solvents. Principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) plots showed significant differences between the diploid and tetraploid metabolite profiles. Extracts of tetraploid showed higher amounts of amino acids, while extracts of diploid contained more organic acids and sugars. [Figure not available: see fulltext.]",

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AU - Gorinstein, Shela

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Identification and Characterization of Diploid and Tetraploid in Platycodon grandiflorum

Abstract

Bibliographical note

Keywords

UN SDGs

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