Identification and Isolation of Chicken Sucrase-Isomaltase cDNA Sequence

Zehava Uni*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

The intestinal sucrase-isomaltase (SI) complex is a glycoprotein of the small intestine brush border membrane that plays an important role in the final degradation of carbohydrate. To clone the chicken SI, we employed reverse transcriptase polymerase chain reaction (RT-PCR). Agarose gel electrophoresis of the PCR products exhibited one amplified band of approximately 800 bp. The fragment was extracted from the gel and sequenced. The cDNA sequence of the chicken SI is 786 bp in length and exhibits 99% identity at the nucleotide level to the Homo sapiens SI mRNA. Using our cDNA as a probe, Northern analysis revealed a transcript of approximately 6.0 kb in chicken jejunum and ileum tissues.

Original languageAmerican English
Pages (from-to)140-144
Number of pages5
JournalPoultry Science
Volume77
Issue number1
DOIs
StatePublished - Jan 1998

Keywords

  • Chicken
  • Nucleotide sequence
  • Polymerase chain reaction
  • Sucrase-isomaltase complex

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