Identification and mapping of PmG16, a powdery mildew resistance gene derived from wild emmer wheat

Roi Ben-David, Weilong Xie, Zvi Peleg, Yehoshua Saranga, Amos Dinoor, Tzion Fahima*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

The gene-pool of wild emmer wheat, Triticum turgidum ssp. dicoccoides, harbors a rich allelic repertoire for disease resistance. In the current study, we made use of tetraploid wheat mapping populations derived from a cross between durum wheat (cv. Langdon) and wild emmer (accession G18-16) to identify and map a new powdery mildew resistance gene derived from wild emmer wheat. Initially, the two parental lines were screened with a collection of 42 isolates of Blumeria graminis f. sp. tritici (Bgt) from Israel and 5 isolates from Switzerland. While G18-16 was resistant to 34 isolates, Langdon was resistant only to 5 isolates and susceptible to 42 isolates. Isolate Bgt#15 was selected to differentiate between the disease reactions of the two genotypes. Segregation ratio of F2-3 and recombinant inbreed line (F7) populations to inoculation with isolate Bgt#15 indicated the role of a single dominant gene in conferring resistance to Bgt#15. This gene, temporarily designated PmG16, was located on the distal region of chromosome arm 7AL. Genetic map of PmG16 region was assembled with 32 simple sequence repeat (SSR), sequence tag site (STS), Diversity array technology (DArT) and cleaved amplified polymorphic sequence (CAPS) markers and assigned to the 7AL physical bin map (7AL-16). Using four DNA markers we established colinearity between the genomic region spanning the PmG16 locus within the distal region of chromosome arm 7AL and the genomic regions on rice chromosome 6 and Brachypodium Bd1. A comparative analysis was carried out between PmG16 and other known Pm genes located on chromosome arm 7AL. The identified PmG16 may facilitate the use of wild alleles for improvement of powdery mildew resistance in elite wheat cultivars via marker-assisted selection.

Original languageAmerican English
Pages (from-to)499-510
Number of pages12
JournalTheoretical And Applied Genetics
Volume121
Issue number3
DOIs
StatePublished - Aug 2010

Bibliographical note

Funding Information:
This work was supported by The Israel Science Foundation grants # 608/03 and 1089/04 and equipment grants #048/99, #1478/04 and #1719/08. The authors thank A. Fahum, M. Chatzav and U. Uner for their excellent technical assistance. The authors thank B. Keller for providing five Swiss Bgt isolates. Z. Peleg is indebted to the Israel Council for Higher Education postdoctoral fellowships award.

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