TY - JOUR
T1 - Identification and mode of inheritance of quantitative trait loci for secondary metabolite abundance in tomato
AU - Alseekh, Saleh
AU - Tohge, Takayuki
AU - Wendenberg, Regina
AU - Scossa, Federico
AU - Omranian, Nooshin
AU - Li, Jie
AU - Kleessen, Sabrina
AU - Giavalisco, Patrick
AU - Pleban, Tzili
AU - Mueller-Roeber, Bernd
AU - Zamir, Dani
AU - Nikoloski, Zoran
AU - Fernie, Alisdair R.
N1 - Publisher Copyright:
© 2015 American Society of Plant Biologists. All rights reserved.
PY - 2015
Y1 - 2015
N2 - A large-scale metabolic quantitative trait loci (mQTL) analysis was performed on the well-characterized Solanum pennellii introgression lines to investigate the genomic regions associated with secondary metabolism in tomato fruit pericarp. In total, 679 mQTLs were detected across the 76 introgression lines. Heritability analyses revealed that mQTLs of secondary metabolism were less affected by environment than mQTLs of primary metabolism. Network analysis allowed us to assess the interconnectivity of primary and secondary metabolism as well as to compare and contrast their respective associations with morphological traits. Additionally, we applied a recently established real-time quantitative PCR platform to gain insight into transcriptional control mechanisms of a subset of the mQTLs, including those for hydroxycinnamates, acyl-sugar, naringenin chalcone, and a range of glycoalkaloids. Intriguingly, many of these compounds displayed a dominant-negative mode of inheritance, which is contrary to the conventional wisdom that secondary metabolite contents decreased on domestication. We additionally performed an exemplary evaluation of two candidate genes for glycolalkaloid mQTLs via the use of virus-induced gene silencing. The combined data of this study were compared with previous results on primary metabolism obtained from the same material and to other studies of natural variance of secondary metabolism.
AB - A large-scale metabolic quantitative trait loci (mQTL) analysis was performed on the well-characterized Solanum pennellii introgression lines to investigate the genomic regions associated with secondary metabolism in tomato fruit pericarp. In total, 679 mQTLs were detected across the 76 introgression lines. Heritability analyses revealed that mQTLs of secondary metabolism were less affected by environment than mQTLs of primary metabolism. Network analysis allowed us to assess the interconnectivity of primary and secondary metabolism as well as to compare and contrast their respective associations with morphological traits. Additionally, we applied a recently established real-time quantitative PCR platform to gain insight into transcriptional control mechanisms of a subset of the mQTLs, including those for hydroxycinnamates, acyl-sugar, naringenin chalcone, and a range of glycoalkaloids. Intriguingly, many of these compounds displayed a dominant-negative mode of inheritance, which is contrary to the conventional wisdom that secondary metabolite contents decreased on domestication. We additionally performed an exemplary evaluation of two candidate genes for glycolalkaloid mQTLs via the use of virus-induced gene silencing. The combined data of this study were compared with previous results on primary metabolism obtained from the same material and to other studies of natural variance of secondary metabolism.
UR - http://www.scopus.com/inward/record.url?scp=84927717970&partnerID=8YFLogxK
U2 - 10.1105/tpc.114.132266
DO - 10.1105/tpc.114.132266
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 25770107
AN - SCOPUS:84927717970
SN - 1040-4651
VL - 27
SP - 485
EP - 512
JO - Plant Cell
JF - Plant Cell
IS - 3
ER -