Identification and nucleotide sequence of Brucella melitensis L7/L12 ribosomal protein

Gilad Bachrach; Dror Bar-Nir; Menachem Banai; Herve Bercovier

doi:10.1111/j.1574-6968.1994.tb07039.x

Identification and nucleotide sequence of Brucella melitensis L7/L12 ribosomal protein

Gilad Bachrach, Dror Bar-Nir, Menachem Banai, Herve Bercovier^*

^*Corresponding author for this work

Institute of Biomedical Research at the Faculty of Dental Medicine

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

DNA sequencing of the gene encoding a Brucella melitensis 12-kDa protein revealed that this protein was the ribosomal protein L7/L12. The B. melitensis L7/L12 DNA sequence was identical to that of the corresponding B. abortus gene, showing the near identity of these two organisms. When comparing the sequence of this protein to that of other organisms some domains were highly conserved, especially the C-terminus, which contrasted with the lack of conservation of the sequences at the N-terminus. The finding that the ribosomal protein L7/L12 of Brucella is an immunodominant antigen provides a new rationale to explain the activity of ribosomal vaccines.

Original language	English
Pages (from-to)	237-240
Number of pages	4
Journal	FEMS Microbiology Letters
Volume	120
Issue number	3
DOIs	https://doi.org/10.1111/j.1574-6968.1994.tb07039.x
State	Published - 15 Jul 1994

Bibliographical note

Funding Information:
This research was supported in part by funds provided by the Agency for International Development under the Middle East regional cooperation program, managed by the US Department of Agriculture, Trinational animal health program and by grant IS-1434-88 from the US-Israel BARD foundation.

Keywords

Brucella melitensis
DNA sequence
L7/L12
Ribosomal protein

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10.1111/j.1574-6968.1994.tb07039.x

Cite this

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title = "Identification and nucleotide sequence of Brucella melitensis L7/L12 ribosomal protein",

abstract = "DNA sequencing of the gene encoding a Brucella melitensis 12-kDa protein revealed that this protein was the ribosomal protein L7/L12. The B. melitensis L7/L12 DNA sequence was identical to that of the corresponding B. abortus gene, showing the near identity of these two organisms. When comparing the sequence of this protein to that of other organisms some domains were highly conserved, especially the C-terminus, which contrasted with the lack of conservation of the sequences at the N-terminus. The finding that the ribosomal protein L7/L12 of Brucella is an immunodominant antigen provides a new rationale to explain the activity of ribosomal vaccines.",

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AU - Bercovier, Herve

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AB - DNA sequencing of the gene encoding a Brucella melitensis 12-kDa protein revealed that this protein was the ribosomal protein L7/L12. The B. melitensis L7/L12 DNA sequence was identical to that of the corresponding B. abortus gene, showing the near identity of these two organisms. When comparing the sequence of this protein to that of other organisms some domains were highly conserved, especially the C-terminus, which contrasted with the lack of conservation of the sequences at the N-terminus. The finding that the ribosomal protein L7/L12 of Brucella is an immunodominant antigen provides a new rationale to explain the activity of ribosomal vaccines.

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Identification and nucleotide sequence of Brucella melitensis L7/L12 ribosomal protein

Abstract

Bibliographical note

Keywords

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