Identification and RNA-binding properties of an initiation factor capable of relieving translational inhibition induced by heme deprivation or double-stranded RNA

Raymond Kaempfer*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

An initiation factor from rabbit reticulocytes can overcome the block in initiation of protein synthesis occurring in reticulocyte lysates when exogenous hemin is not present, or when double-stranded RNA is added. This factor has been identified with IF-MP, an initiation factor capable of forming ternary complexes with GTP and methionyl-tRNAF. Initiation factor IF-M3 by itself is unable to overcome the block in initiation, but appears to stimulate this activity of IF-MP. IF-MP binds to single-stranded R17 RNA as well as to double-stranded RNA, while IF-M3 only binds to double-stranded RNA. The protein synthetic activity of IF-MP is sensitive to N-ethylmaleimide, but its ability to bind RNA is resistant.

Original languageEnglish
Pages (from-to)591-597
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume61
Issue number2
DOIs
StatePublished - 27 Nov 1974
Externally publishedYes

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