TY - JOUR
T1 - Identification of 11,13-dihydroxy-14-octadecaenoic acid as a circulating Na+/K+-ATPase inhibitor
AU - Lichtstein, D.
AU - Samuelov, S.
AU - Gati, I.
AU - Felix, A. M.
AU - Gabriel, T. F.
AU - Deutsch, J.
PY - 1991
Y1 - 1991
N2 - Two digitalis-like compounds (DLC) were purified to homogeneity from bovine plasma. The purification procedure consisted of organic extractions and batch chromatography followed by three subsequent separations using reverse-phase high-performance liquid chromatography. The presence of a DLC in the different fractions was monitored by their ability to inhibit (a) [3H]ouabain binding to rat brain synaptosomes, and (b) microsomal Na+/K+-ATPase activity. Using mass spectrometry and nuclear magnetic resonance spectroscopy the structure of one of the DLCs was identified as 11,13-dihydroxy-14-octadecaenoic acid. It is suggested that this new hydroxy, unsaturated, fatty acid derivative may regulate Na+/K+-ATPase activity under some physiological and pathological conditions.
AB - Two digitalis-like compounds (DLC) were purified to homogeneity from bovine plasma. The purification procedure consisted of organic extractions and batch chromatography followed by three subsequent separations using reverse-phase high-performance liquid chromatography. The presence of a DLC in the different fractions was monitored by their ability to inhibit (a) [3H]ouabain binding to rat brain synaptosomes, and (b) microsomal Na+/K+-ATPase activity. Using mass spectrometry and nuclear magnetic resonance spectroscopy the structure of one of the DLCs was identified as 11,13-dihydroxy-14-octadecaenoic acid. It is suggested that this new hydroxy, unsaturated, fatty acid derivative may regulate Na+/K+-ATPase activity under some physiological and pathological conditions.
UR - http://www.scopus.com/inward/record.url?scp=0025962266&partnerID=8YFLogxK
U2 - 10.1677/joe.0.1280071
DO - 10.1677/joe.0.1280071
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 1847965
AN - SCOPUS:0025962266
SN - 0022-0795
VL - 128
SP - 71
EP - 78
JO - Journal of Endocrinology
JF - Journal of Endocrinology
IS - 1
ER -