Identification of 11,13-dihydroxy-14-octadecaenoic acid as a circulating Na+/K+-ATPase inhibitor

D. Lichtstein, S. Samuelov, I. Gati, A. M. Felix, T. F. Gabriel, J. Deutsch

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Abstract

Two digitalis-like compounds (DLC) were purified to homogeneity from bovine plasma. The purification procedure consisted of organic extractions and batch chromatography followed by three subsequent separations using reverse-phase high-performance liquid chromatography. The presence of a DLC in the different fractions was monitored by their ability to inhibit (a) [3H]ouabain binding to rat brain synaptosomes, and (b) microsomal Na+/K+-ATPase activity. Using mass spectrometry and nuclear magnetic resonance spectroscopy the structure of one of the DLCs was identified as 11,13-dihydroxy-14-octadecaenoic acid. It is suggested that this new hydroxy, unsaturated, fatty acid derivative may regulate Na+/K+-ATPase activity under some physiological and pathological conditions.

Original languageEnglish
Pages (from-to)71-78
Number of pages8
JournalJournal of Endocrinology
Volume128
Issue number1
DOIs
StatePublished - 1991

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