TY - JOUR
T1 - Identification of a tau promoter region mediating tissue-specific-regulated expression in PC12 cells
AU - Sadot, Einat
AU - Heicklen-Klein, Alice
AU - Barg, Jacob
AU - Lazarovici, Philip
AU - Ginzburg, Irith
PY - 1996/3/15
Y1 - 1996/3/15
N2 - Tau, a microtubule-associated protein, is encoded by a single gene, the expression of which is neuron-specific and developmentally regulated. When PC12 cells are exposed to nerve growth factor (NGF), they differentiate to sympathetic-like neurons. This differentiation process is accompanied by an elevation of tau proteins and mRNA. Here, we describe, for the first time, the isolation and characterization of a tau promoter region. We show that the promoter of tau is G + C-rich, lacks a genuine TATA box and thus promotes multiple initiation sites of RNA transcription. Our results demonstrate that a region of approximately 335 base-pairs residing immediately upstream of tau exon -1 are able to direct positive control of neuron-specific activity of the luciferase reporter gene. The isolation of tau promoter will facilitate further studies of the regulation of tau expression during development and aging of neuronal cells.
AB - Tau, a microtubule-associated protein, is encoded by a single gene, the expression of which is neuron-specific and developmentally regulated. When PC12 cells are exposed to nerve growth factor (NGF), they differentiate to sympathetic-like neurons. This differentiation process is accompanied by an elevation of tau proteins and mRNA. Here, we describe, for the first time, the isolation and characterization of a tau promoter region. We show that the promoter of tau is G + C-rich, lacks a genuine TATA box and thus promotes multiple initiation sites of RNA transcription. Our results demonstrate that a region of approximately 335 base-pairs residing immediately upstream of tau exon -1 are able to direct positive control of neuron-specific activity of the luciferase reporter gene. The isolation of tau promoter will facilitate further studies of the regulation of tau expression during development and aging of neuronal cells.
KW - EGF
KW - Neuronal promoter
KW - NGF
KW - PC12 cells
KW - Tau
UR - http://www.scopus.com/inward/record.url?scp=0029933181&partnerID=8YFLogxK
U2 - 10.1006/jmbi.1996.0126
DO - 10.1006/jmbi.1996.0126
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C2 - 8601831
AN - SCOPUS:0029933181
SN - 0022-2836
VL - 256
SP - 805
EP - 812
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 5
ER -