Identification of bloodmeals in wild caught blood fed Phlebotomus argentipes (Diptera: Psychodidae) using cytochrome b PCR and reverse line blotting in Bihar, India

Rajesh B. Garlapati, Ibrahim Abbasi, Alon Warburg, David Poché, Richard Poché*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Identification of the source of bloodmeals in vectors plays an important role in epidemiological studies by determining the host preferences of wild sand flies in natural habitat. The anthropophilic index is a crucial component in human leishmaniasis. Bloodmeal analysis can identify the reservoir hosts of vector borne diseases. The amplification of the mitochondrial cytochrome b gene, followed by reverse line blot analysis, helps to identify the bloodmeal ingested by the wild caught sand flies. In the current study, blood fed sand flies were collected from three different villages in Bihar, India, by using Centers for Disease Control mini traps with incandescent light. Traps were placed in five different sites in the villages. Whole genome DNA was extracted from the blood fed sand flies and was amplified for the cytochrome b region, followed by reverse line blot analysis. In total, 442 blood fed sand flies were analyzed out of which 288 (65%) were positive to cytochrome b polymerase chain reaction. Humans, cattle, buffalo, and goats were the major bloodmeals identified, followed by chickens. In some of the blood fed sand flies, multiple bloodmeals were identified. In the current study, sand flies mostly fed on humans, followed by cattle, buffalo, and goats. In this regard, it is necessary to also consider cattle, buffalo, and goats when addressing vector control in Bihar, India.

Original languageAmerican English
Pages (from-to)515-521
Number of pages7
JournalJournal of Medical Entomology
Volume49
Issue number3
DOIs
StatePublished - May 2012

Keywords

  • bloodmeal identification
  • cytochrome b PCR
  • host
  • sand fly
  • visceral leishmaniasis

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