Identification of germline transcriptional regulatory elements in Aedes aegypti

Omar S. Akbari, Philippos A. Papathanos, Jeremy E. Sandler, Katie Kennedy, Bruce A. Hay*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

The mosquito Aedes aegypti is the principal vector for the yellow fever and dengue viruses, and is also responsible for recent outbreaks of the alphavirus chikungunya. Vector control strategies utilizing engineered gene drive systems are being developed as a means of replacing wild, pathogen transmitting mosquitoes with individuals refractory to disease transmission, or bringing about population suppression. Several of these systems, including Medea, UDMEL, and site-specific nucleases, which can be used to drive genes into populations or bring about population suppression, utilize transcriptional regulatory elements that drive germline-specific expression. Here we report the identification of multiple regulatory elements able to drive gene expression specifically in the female germline, or in the male and female germline, in the mosquito Aedes aegypti. These elements can also be used as tools with which to probe the roles of specific genes in germline function and in the early embryo, through overexpression or RNA interference.

Original languageAmerican English
Article number3954
JournalScientific Reports
Volume4
DOIs
StatePublished - 1 Apr 2014
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2014, Nature Publishing Group. All rights reserved.

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