Identification of gold nanoparticle-resistant mutants of Saccharomyces cerevisiae suggests a role for respiratory metabolism in mediating toxicity

Mark R. Smith; Matthew G. Boenzli; Vihangi Hindagolla; Jun Ding; John M. Miller; James E. Hutchison; Jeffrey A. Greenwood; Hagai Abeliovich; Alan T. Bakalinsky

doi:10.1128/AEM.01737-12

Identification of gold nanoparticle-resistant mutants of Saccharomyces cerevisiae suggests a role for respiratory metabolism in mediating toxicity

Mark R. Smith, Matthew G. Boenzli, Vihangi Hindagolla, Jun Ding, John M. Miller, James E. Hutchison, Jeffrey A. Greenwood, Hagai Abeliovich, Alan T. Bakalinsky^*

^*Corresponding author for this work

Institute of Biochemistry, Food Science and Nutrition

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Positively charged gold nanoparticles (0.8-nm core diameter) reduced yeast survival, but not growth, at a concentration of 10 to 100 μg/ml. Among 17 resistant deletion mutants isolated in a genome-wide screen, highly significant enrichment was observed for respiration-deficient mutants lacking genes encoding proteins associated with the mitochondrion.

Original language	English
Pages (from-to)	728-733
Number of pages	6
Journal	Applied and Environmental Microbiology
Volume	79
Issue number	2
DOIs	https://doi.org/10.1128/AEM.01737-12
State	Published - Jan 2013

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Smith, M. R., Boenzli, M. G., Hindagolla, V., Ding, J., Miller, J. M., Hutchison, J. E., Greenwood, J. A., Abeliovich, H., & Bakalinsky, A. T. (2013). Identification of gold nanoparticle-resistant mutants of Saccharomyces cerevisiae suggests a role for respiratory metabolism in mediating toxicity. Applied and Environmental Microbiology, 79(2), 728-733. https://doi.org/10.1128/AEM.01737-12

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abstract = "Positively charged gold nanoparticles (0.8-nm core diameter) reduced yeast survival, but not growth, at a concentration of 10 to 100 μg/ml. Among 17 resistant deletion mutants isolated in a genome-wide screen, highly significant enrichment was observed for respiration-deficient mutants lacking genes encoding proteins associated with the mitochondrion.",

author = "Smith, {Mark R.} and Boenzli, {Matthew G.} and Vihangi Hindagolla and Jun Ding and Miller, {John M.} and Hutchison, {James E.} and Greenwood, {Jeffrey A.} and Hagai Abeliovich and Bakalinsky, {Alan T.}",

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Smith, MR, Boenzli, MG, Hindagolla, V, Ding, J, Miller, JM, Hutchison, JE, Greenwood, JA, Abeliovich, H & Bakalinsky, AT 2013, 'Identification of gold nanoparticle-resistant mutants of Saccharomyces cerevisiae suggests a role for respiratory metabolism in mediating toxicity', Applied and Environmental Microbiology, vol. 79, no. 2, pp. 728-733. https://doi.org/10.1128/AEM.01737-12

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AU - Smith, Mark R.

AU - Boenzli, Matthew G.

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AU - Ding, Jun

AU - Miller, John M.

AU - Hutchison, James E.

AU - Greenwood, Jeffrey A.

AU - Abeliovich, Hagai

AU - Bakalinsky, Alan T.

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AB - Positively charged gold nanoparticles (0.8-nm core diameter) reduced yeast survival, but not growth, at a concentration of 10 to 100 μg/ml. Among 17 resistant deletion mutants isolated in a genome-wide screen, highly significant enrichment was observed for respiration-deficient mutants lacking genes encoding proteins associated with the mitochondrion.

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Identification of gold nanoparticle-resistant mutants of Saccharomyces cerevisiae suggests a role for respiratory metabolism in mediating toxicity

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