Identification of QTLs associated with spring vegetative budbreak time after dormancy release in pear (Pyrus communis L.)

Gilad Gabay; Yardena Dahan; Yacov Izhaki; Tal Isaacson; Yonatan Elkind; Giora Ben-Ari; Moshe A. Flaishman

doi:10.1111/pbr.12499

Identification of QTLs associated with spring vegetative budbreak time after dormancy release in pear (Pyrus communis L.)

Gilad Gabay, Yardena Dahan, Yacov Izhaki, Tal Isaacson, Yonatan Elkind, Giora Ben-Ari, Moshe A. Flaishman^*

^*Corresponding author for this work

Institute of Plant Sciences and Genetics in Agriculture

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Dormancy release is greatly affected by chilling unit (CU) accumulation. Lack of CU has a major impact on spring vegetative budbreak (VB). To understand the genetic mechanism governing the chilling requirement (CR), we conducted a QTL analysis of VB date in F₁ population, derived from a cross between ‘Spadona’ (low CR) and ‘Harrow Sweet’ (high CR). Using a unique methodology of tree mobility, replicates of the same genotypes were exposed during the winter, over two consecutive years, to climates that differ greatly in their CU and to the same heat conditions to induce VB, in order to evaluate CR genetic impact and to distinguish it from the heat factor. Broad-sense heritability within locations ranged from 0.62 to 0.66. Due to a strong impact of GxE interaction, it was reduced to 0.46 for the overall mean. We examined the previously discovered apple QTLs detected in linkage groups (LG) 9 and 8, based on the synteny between the species. Our analysis confirms significant QTLs in LG8 (R² = 12%–24%) and LG9 (R² = 20%–38%) for all locations and years.

Original language	American English
Pages (from-to)	749-758
Number of pages	10
Journal	Plant Breeding
Volume	136
Issue number	5
DOIs	https://doi.org/10.1111/pbr.12499
State	Published - Oct 2017

Bibliographical note

Funding Information:
We would like to thank Dr. Assaf Distelfeld from the Tel Aviv University for use of the equipment to evaluate SSR marker polymorphism. We would also like to thank Dr. Sigal Popovsky-Sarid for her help with genetic map positioning and QTL mapping. Funding was provided by the Ministry of Agriculture, Bet Dagan, Israel.

Publisher Copyright:
© 2017 Blackwell Verlag GmbH

Keywords

chilling requirement
chilling units
genotype x environment interaction
linkage group
marker-assisted selection

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10.1111/pbr.12499

Cite this

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title = "Identification of QTLs associated with spring vegetative budbreak time after dormancy release in pear (Pyrus communis L.)",

abstract = "Dormancy release is greatly affected by chilling unit (CU) accumulation. Lack of CU has a major impact on spring vegetative budbreak (VB). To understand the genetic mechanism governing the chilling requirement (CR), we conducted a QTL analysis of VB date in F1 population, derived from a cross between {\textquoteleft}Spadona{\textquoteright} (low CR) and {\textquoteleft}Harrow Sweet{\textquoteright} (high CR). Using a unique methodology of tree mobility, replicates of the same genotypes were exposed during the winter, over two consecutive years, to climates that differ greatly in their CU and to the same heat conditions to induce VB, in order to evaluate CR genetic impact and to distinguish it from the heat factor. Broad-sense heritability within locations ranged from 0.62 to 0.66. Due to a strong impact of GxE interaction, it was reduced to 0.46 for the overall mean. We examined the previously discovered apple QTLs detected in linkage groups (LG) 9 and 8, based on the synteny between the species. Our analysis confirms significant QTLs in LG8 (R2 = 12%–24%) and LG9 (R2 = 20%–38%) for all locations and years.",

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author = "Gilad Gabay and Yardena Dahan and Yacov Izhaki and Tal Isaacson and Yonatan Elkind and Giora Ben-Ari and Flaishman, {Moshe A.}",

note = "Funding Information: We would like to thank Dr. Assaf Distelfeld from the Tel Aviv University for use of the equipment to evaluate SSR marker polymorphism. We would also like to thank Dr. Sigal Popovsky-Sarid for her help with genetic map positioning and QTL mapping. Funding was provided by the Ministry of Agriculture, Bet Dagan, Israel. Publisher Copyright: {\textcopyright} 2017 Blackwell Verlag GmbH",

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AU - Isaacson, Tal

AU - Elkind, Yonatan

AU - Ben-Ari, Giora

AU - Flaishman, Moshe A.

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N2 - Dormancy release is greatly affected by chilling unit (CU) accumulation. Lack of CU has a major impact on spring vegetative budbreak (VB). To understand the genetic mechanism governing the chilling requirement (CR), we conducted a QTL analysis of VB date in F1 population, derived from a cross between ‘Spadona’ (low CR) and ‘Harrow Sweet’ (high CR). Using a unique methodology of tree mobility, replicates of the same genotypes were exposed during the winter, over two consecutive years, to climates that differ greatly in their CU and to the same heat conditions to induce VB, in order to evaluate CR genetic impact and to distinguish it from the heat factor. Broad-sense heritability within locations ranged from 0.62 to 0.66. Due to a strong impact of GxE interaction, it was reduced to 0.46 for the overall mean. We examined the previously discovered apple QTLs detected in linkage groups (LG) 9 and 8, based on the synteny between the species. Our analysis confirms significant QTLs in LG8 (R2 = 12%–24%) and LG9 (R2 = 20%–38%) for all locations and years.

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Identification of QTLs associated with spring vegetative budbreak time after dormancy release in pear (Pyrus communis L.)

Abstract

Bibliographical note

Keywords

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