Identification of salivary protein biomarkers for orthodontically induced inflammatory root resorption

Karolina Elżbieta Kaczor-Urbanowicz*, Omer Deutsch, Batia Zaks, Guy Krief, Stella Chaushu, Aaron Palmon

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


Purpose: Orthodontically induced inflammatory root resorption (OIIRR) is one of the most prevalent and unavoidable consequence of orthodontic tooth movement. The aim of this study was to discover potential diagnostic protein biomarkers for detection of OIIRR in whole saliva (WS). Material and methods: Unstimulated WS was collected from 72 subjects: 48 OIIRR patients and 24 untreated, generally healthy, age and gender matched controls. Radiographic assessment of periapical x-rays of four upper incisors taken before and 9 months after bonding was done. High-abundance proteins were depleted followed by two-dimensional-gel-electrophoresis and quantitative mass spectrometry (qMS). Finally, to initially validate qMS results, Western blotting was performed. Results: qMS revealed differentially expressed proteins in the moderate-to-severe OIIRR group, which have never been found in WS before. Additionally, in the moderate-to-severe young OIIRR group, the pathogenetic mechanisms related to actin cytoskeleton regulation and Fc gamma R- mediated phagocytosis were detected, while in adults- to focal adhesion. Preliminary validation by Western blotting of fetuin-A and p21-ARC indicated expression profile trends similar to those identified by qMS. Conclusion: The significance of WS novel proteomic methodologies is clearly demonstrated for detecting new OIIRR biomarkers as well as for unveiling possible novel pathogenetic mechanisms in both young and adult patients.

Original languageAmerican English
Article number1600119
JournalProteomics - Clinical Applications
Issue number9-10
StatePublished - Sep 2017

Bibliographical note

Publisher Copyright:
© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim


  • Biomarkers
  • Mass spectrometry
  • Orthodontic treatment
  • Root resorption
  • Saliva


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