TY - JOUR
T1 - Identification of snails infected with schistosomes by ELISA employing monoclonal antibodies
T2 - Schistosoma mansoni in laboratory snails (Biomphalaria glabrata) and in field snails (Biomphalaria pfeifereri) from Kenya
AU - Hamburger, J.
AU - Weil, M.
AU - Turetzky, T.
AU - Ouma, J. H.
AU - Koech, D. K.
AU - Klumpp, R.
AU - Siongok, T. K.A.
AU - Sturrock, R. F.
PY - 1989
Y1 - 1989
N2 - An enzyme-linked immusorbent assay (ELISA) employing monoclonal antibodies was used for detecting Schistosoma mansoni antigens in hemolymph of laboratory snails (Biomphalaria glabrata) in Kenya. Infected laboratory snails shedding cercariae were differentially identified by ELISA from uninfected snails with 100% sensitivity and specificity. Prepatent infections were detected by ELISA from 2 weeks after exposure to miracidia. Thus, ELISA revealed infection 3 weeks before maximal patency was reached (5-6 weeks post-exposure). Infected field snails (B. pfeifferi) shedding cercariae were differentially identified by ELISA, with 100% sensitivity and specificity from uninfected field snails and from snails naturally infected with other trematodes (echinostomes and strigeids). Prepatent infections with S. mansoni were readily identified by ELISA in field snails. A case is demonstrated where infection rate, as determined by shedding test alone, was 9.8%, whereas the combined figure of prepatent and patient infection rates was 22.9%.
AB - An enzyme-linked immusorbent assay (ELISA) employing monoclonal antibodies was used for detecting Schistosoma mansoni antigens in hemolymph of laboratory snails (Biomphalaria glabrata) in Kenya. Infected laboratory snails shedding cercariae were differentially identified by ELISA from uninfected snails with 100% sensitivity and specificity. Prepatent infections were detected by ELISA from 2 weeks after exposure to miracidia. Thus, ELISA revealed infection 3 weeks before maximal patency was reached (5-6 weeks post-exposure). Infected field snails (B. pfeifferi) shedding cercariae were differentially identified by ELISA, with 100% sensitivity and specificity from uninfected field snails and from snails naturally infected with other trematodes (echinostomes and strigeids). Prepatent infections with S. mansoni were readily identified by ELISA in field snails. A case is demonstrated where infection rate, as determined by shedding test alone, was 9.8%, whereas the combined figure of prepatent and patient infection rates was 22.9%.
UR - http://www.scopus.com/inward/record.url?scp=0024346555&partnerID=8YFLogxK
U2 - 10.4269/ajtmh.1989.40.613
DO - 10.4269/ajtmh.1989.40.613
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 2742038
AN - SCOPUS:0024346555
SN - 0002-9637
VL - 40
SP - 613
EP - 619
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
IS - 6
ER -