TY - JOUR
T1 - Identification of the algal dimethyl sulfide-releasing enzyme
T2 - A missing link in the marine sulfur cycle
AU - Alcolombri, Uria
AU - Ben-Dor, Shifra
AU - Feldmesser, Ester
AU - Levin, Yishai
AU - Tawfik, Dan S.
AU - Vardi, Assaf
N1 - Publisher Copyright:
© 2015, American Association for the Advancement of Science. All rights reserved.
PY - 2015/6/26
Y1 - 2015/6/26
N2 - Algal blooms produce large amounts of dimethyl sulfide (DMS), a volatile with a diverse signaling role in marine food webs that is emitted to the atmosphere, where it can affect cloud formation. The algal enzymes responsible for forming DMS from dimethylsulfoniopropionate (DMSP) remain unidentified despite their critical role in the global sulfur cycle. We identified and characterized Alma1, a DMSP lyase from the bloom-forming algae Emiliania huxleyi. Alma1 is a tetrameric, redox-sensitive enzyme of the aspartate racemase superfamily. Recombinant Alma1 exhibits biochemical features identical to the DMSP lyase in E. huxleyi, and DMS released by various E. huxleyi isolates correlates with their Alma1 levels. Sequence homology searches suggest that Alma1 represents a gene family present in major, globally distributed phytoplankton taxa and in other marine organisms.
AB - Algal blooms produce large amounts of dimethyl sulfide (DMS), a volatile with a diverse signaling role in marine food webs that is emitted to the atmosphere, where it can affect cloud formation. The algal enzymes responsible for forming DMS from dimethylsulfoniopropionate (DMSP) remain unidentified despite their critical role in the global sulfur cycle. We identified and characterized Alma1, a DMSP lyase from the bloom-forming algae Emiliania huxleyi. Alma1 is a tetrameric, redox-sensitive enzyme of the aspartate racemase superfamily. Recombinant Alma1 exhibits biochemical features identical to the DMSP lyase in E. huxleyi, and DMS released by various E. huxleyi isolates correlates with their Alma1 levels. Sequence homology searches suggest that Alma1 represents a gene family present in major, globally distributed phytoplankton taxa and in other marine organisms.
UR - http://www.scopus.com/inward/record.url?scp=84933556353&partnerID=8YFLogxK
U2 - 10.1126/science.aab1586
DO - 10.1126/science.aab1586
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C2 - 26113722
AN - SCOPUS:84933556353
SN - 0036-8075
VL - 348
SP - 1466
EP - 1469
JO - Science
JF - Science
IS - 6242
ER -