Identification of the causative agent of cutaneous leishmaniasis in Chichaoua province, Morocco

M Rhajaoui; F Sebti; H Fellah; M Z Alam; A Nasereddin; Ibrahim Abbasi; G Schönian

doi:10.1051/parasite/2012191081

Identification of the causative agent of cutaneous leishmaniasis in Chichaoua province, Morocco

M Rhajaoui, F Sebti, H Fellah, M Z Alam, A Nasereddin, Ibrahim Abbasi, G Schönian

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Cutaneous leishmaniasis (CL) in Morocco is caused by three species, Leishmania major, L. tropica and L. infantum. CL has been known in Chichaoua province since 2000. Using DNA extracted from microscopic slides and parasite cultures, collected in the years 2006 and 2009, we identified for the first time L. tropica as the causative agent of CL in this region. Species identification was achieved by performing the ITS1-PCR-RFLP approach. By using this method it was possible to identify parasites in Giemsa stained slides containing less than five parasites per oil-immersion field even they were conserved for up to four months.

Original language	American English
Pages (from-to)	81-84
Number of pages	4
Journal	Parasite
Volume	19
Issue number	1
DOIs	https://doi.org/10.1051/parasite/2012191081
State	Published - Feb 2012
Externally published	Yes

Keywords

DNA, Protozoan/isolation & purification
DNA, Ribosomal Spacer/isolation & purification
Humans
Leishmania tropica/genetics
Leishmaniasis, Cutaneous/parasitology
Morocco
Polymerase Chain Reaction/methods
Polymorphism, Restriction Fragment Length

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10.1051/parasite/2012191081

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title = "Identification of the causative agent of cutaneous leishmaniasis in Chichaoua province, Morocco",

abstract = "Cutaneous leishmaniasis (CL) in Morocco is caused by three species, Leishmania major, L. tropica and L. infantum. CL has been known in Chichaoua province since 2000. Using DNA extracted from microscopic slides and parasite cultures, collected in the years 2006 and 2009, we identified for the first time L. tropica as the causative agent of CL in this region. Species identification was achieved by performing the ITS1-PCR-RFLP approach. By using this method it was possible to identify parasites in Giemsa stained slides containing less than five parasites per oil-immersion field even they were conserved for up to four months.",

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AU - Rhajaoui, M

AU - Sebti, F

AU - Fellah, H

AU - Alam, M Z

AU - Nasereddin, A

AU - Abbasi, Ibrahim

AU - Schönian, G

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AB - Cutaneous leishmaniasis (CL) in Morocco is caused by three species, Leishmania major, L. tropica and L. infantum. CL has been known in Chichaoua province since 2000. Using DNA extracted from microscopic slides and parasite cultures, collected in the years 2006 and 2009, we identified for the first time L. tropica as the causative agent of CL in this region. Species identification was achieved by performing the ITS1-PCR-RFLP approach. By using this method it was possible to identify parasites in Giemsa stained slides containing less than five parasites per oil-immersion field even they were conserved for up to four months.

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KW - DNA, Ribosomal Spacer/isolation & purification

KW - Humans

KW - Leishmania tropica/genetics

KW - Leishmaniasis, Cutaneous/parasitology

KW - Morocco

KW - Polymerase Chain Reaction/methods

KW - Polymorphism, Restriction Fragment Length

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EP - 84

JO - Parasite

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Identification of the causative agent of cutaneous leishmaniasis in Chichaoua province, Morocco

Abstract

Keywords

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