IFNG-AS1 Enhances Interferon Gamma Production in Human Natural Killer Cells

Natan Stein, Orit Berhani, Dominik Schmiedel, Alexandra Duev-Cohen, Einat Seidel, Inbal Kol, Pinchas Tsukerman, Merav Hecht, Adi Reches, Moriya Gamliel, Akram Obeidat, Yoav Charpak-Amikam, Rachel Yamin, Ofer Mandelboim*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Long, non-coding RNAs (lncRNAs)are involved in the regulation of many cellular processes. The lncRNA IFNG-AS1 was found to strongly influence the responses to several pathogens in mice by increasing interferon gamma (IFNγ)secretion. Studies have looked at IFNG-AS1 in T cells, yet IFNG-AS1 function in natural killer cells (NKs), an important source of IFNγ, remains unknown. Here, we show a previously undescribed sequence of IFNG-AS1 and report that it may be more abundant in cells than previously thought. Using primary human NKs and an NK line with IFNG-AS1 overexpression, we show that IFNG-AS1 is quickly induced upon NK cell activation, and that IFNG-AS1 overexpression leads to increased IFNγ secretion. Taken together, our work expands IFNG-AS1's activity to the innate arm of the type I immune response, helping to explain its notable effect in animal models of disease.

Original languageAmerican English
Pages (from-to)466-473
Number of pages8
JournaliScience
Volume11
DOIs
StatePublished - 25 Jan 2019

Bibliographical note

Publisher Copyright:
© 2019 The Authors

Keywords

  • Immune Response
  • Immunology
  • Molecular Biology
  • Molecular Mechanism of Gene Regulation

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