IFNG-AS1 Enhances Interferon Gamma Production in Human Natural Killer Cells

Natan Stein; Orit Berhani; Dominik Schmiedel; Alexandra Duev-Cohen; Einat Seidel; Inbal Kol; Pinchas Tsukerman; Merav Hecht; Adi Reches; Moriya Gamliel; Akram Obeidat; Yoav Charpak-Amikam; Rachel Yamin; Ofer Mandelboim

doi:10.1016/j.isci.2018.12.034

IFNG-AS1 Enhances Interferon Gamma Production in Human Natural Killer Cells

Natan Stein, Orit Berhani, Dominik Schmiedel, Alexandra Duev-Cohen, Einat Seidel, Inbal Kol, Pinchas Tsukerman, Merav Hecht, Adi Reches, Moriya Gamliel, Akram Obeidat, Yoav Charpak-Amikam, Rachel Yamin, Ofer Mandelboim^*

^*Corresponding author for this work

Department of Immunology and Cancer Research

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

Long, non-coding RNAs (lncRNAs)are involved in the regulation of many cellular processes. The lncRNA IFNG-AS1 was found to strongly influence the responses to several pathogens in mice by increasing interferon gamma (IFNγ)secretion. Studies have looked at IFNG-AS1 in T cells, yet IFNG-AS1 function in natural killer cells (NKs), an important source of IFNγ, remains unknown. Here, we show a previously undescribed sequence of IFNG-AS1 and report that it may be more abundant in cells than previously thought. Using primary human NKs and an NK line with IFNG-AS1 overexpression, we show that IFNG-AS1 is quickly induced upon NK cell activation, and that IFNG-AS1 overexpression leads to increased IFNγ secretion. Taken together, our work expands IFNG-AS1's activity to the innate arm of the type I immune response, helping to explain its notable effect in animal models of disease.

Original language	English
Pages (from-to)	466-473
Number of pages	8
Journal	iScience
Volume	11
DOIs	https://doi.org/10.1016/j.isci.2018.12.034
State	Published - 25 Jan 2019

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Publisher Copyright:
© 2019 The Authors

Keywords

Immune Response
Immunology
Molecular Biology
Molecular Mechanism of Gene Regulation

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abstract = "Long, non-coding RNAs (lncRNAs)are involved in the regulation of many cellular processes. The lncRNA IFNG-AS1 was found to strongly influence the responses to several pathogens in mice by increasing interferon gamma (IFNγ)secretion. Studies have looked at IFNG-AS1 in T cells, yet IFNG-AS1 function in natural killer cells (NKs), an important source of IFNγ, remains unknown. Here, we show a previously undescribed sequence of IFNG-AS1 and report that it may be more abundant in cells than previously thought. Using primary human NKs and an NK line with IFNG-AS1 overexpression, we show that IFNG-AS1 is quickly induced upon NK cell activation, and that IFNG-AS1 overexpression leads to increased IFNγ secretion. Taken together, our work expands IFNG-AS1's activity to the innate arm of the type I immune response, helping to explain its notable effect in animal models of disease.",

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AU - Berhani, Orit

AU - Schmiedel, Dominik

AU - Duev-Cohen, Alexandra

AU - Seidel, Einat

AU - Kol, Inbal

AU - Tsukerman, Pinchas

AU - Hecht, Merav

AU - Reches, Adi

AU - Gamliel, Moriya

AU - Obeidat, Akram

AU - Charpak-Amikam, Yoav

AU - Yamin, Rachel

AU - Mandelboim, Ofer

PY - 2019/1/25

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N2 - Long, non-coding RNAs (lncRNAs)are involved in the regulation of many cellular processes. The lncRNA IFNG-AS1 was found to strongly influence the responses to several pathogens in mice by increasing interferon gamma (IFNγ)secretion. Studies have looked at IFNG-AS1 in T cells, yet IFNG-AS1 function in natural killer cells (NKs), an important source of IFNγ, remains unknown. Here, we show a previously undescribed sequence of IFNG-AS1 and report that it may be more abundant in cells than previously thought. Using primary human NKs and an NK line with IFNG-AS1 overexpression, we show that IFNG-AS1 is quickly induced upon NK cell activation, and that IFNG-AS1 overexpression leads to increased IFNγ secretion. Taken together, our work expands IFNG-AS1's activity to the innate arm of the type I immune response, helping to explain its notable effect in animal models of disease.

AB - Long, non-coding RNAs (lncRNAs)are involved in the regulation of many cellular processes. The lncRNA IFNG-AS1 was found to strongly influence the responses to several pathogens in mice by increasing interferon gamma (IFNγ)secretion. Studies have looked at IFNG-AS1 in T cells, yet IFNG-AS1 function in natural killer cells (NKs), an important source of IFNγ, remains unknown. Here, we show a previously undescribed sequence of IFNG-AS1 and report that it may be more abundant in cells than previously thought. Using primary human NKs and an NK line with IFNG-AS1 overexpression, we show that IFNG-AS1 is quickly induced upon NK cell activation, and that IFNG-AS1 overexpression leads to increased IFNγ secretion. Taken together, our work expands IFNG-AS1's activity to the innate arm of the type I immune response, helping to explain its notable effect in animal models of disease.

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KW - Molecular Biology

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IFNG-AS1 Enhances Interferon Gamma Production in Human Natural Killer Cells

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Keywords

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