IgE-dependent activation of human mast cells and fMLP-mediated activation of human eosinophils is controlled by the circadian clock

Anja Baumann, Katharina Feilhauer, Stephan C. Bischoff, Oren Froy, Axel Lorentz*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


Symptoms of allergic attacks frequently exhibit diurnal variations. Accordingly, we could recently demonstrate that mast cells and eosinophils - known as major effector cells of allergic diseases - showed an intact circadian clock. Here, we analyzed the role of the circadian clock in the functionality of mast cells and eosinophils. Human intestinal mast cells (hiMC) were isolated from intestinal mucosa; human eosinophils were isolated from peripheral blood. HiMC and eosinophils were synchronized by dexamethasone before stimulation every 4. h around the circadian cycle by FcεRI crosslinking or fMLP, respectively. Signaling molecule activation was examined using Western blot, mRNA expression by real-time RT-PCR, and mediator release by multiplex analysis. CXCL8 and CCL2 were expressed and released in a circadian manner by both hiMC and eosinophils in response to activation. Moreover, phosphorylation of ERK1/2, known to be involved in activation of hiMC and eosinophils, showed circadian rhythms in both cell types. Interestingly, all clock genes hPer1, hPer2, hCry1, hBmal1, and hClock were expressed in a similar circadian pattern in activated and unstimulated cells indicating that the local clock controls hiMC and eosinophils and subsequently allergic reactions but not vice versa.

Original languageAmerican English
Pages (from-to)76-81
Number of pages6
JournalMolecular Immunology
Issue number1
StatePublished - 1 Mar 2015

Bibliographical note

Funding Information:
This work was supported by the Deutsche Forschungsgemeinschaft ( LO 581/7-1 to Axel Lorentz and Oren Froy). The authors thank Yvonne Soltow for excellent technical assistance.

Publisher Copyright:
© 2014 Elsevier Ltd.


  • Allergy
  • Circadian clock
  • Eosinophils
  • Mast cells


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