Abstract
Interleukin 3 (IL-3) promotes the survival and proliferation of hematopoletlc cells of various lineages In culture. Like most other hematopoletlc colony stimulating factors, its mode of action Is unknown. However, binding of the lymphoklneinduces protein tyroslne phosphorylation and enhanced glucose transport In some myelold progenitor cells. We have studiedthe hexose uptake following IL-3 stimulation In IL-3-dependent pro-B cells. IL-3 facilitated the uptake of 2-deoxyglucosewithin 15 min. Kinetic analysis of the 2-deoxyglucose uptake attributed the enhanced transport to improved transporter function, while other hormones or cytokines affect glucose transport primarily via the number of cell surface transporters.
| Original language | English |
|---|---|
| Pages (from-to) | 827-831 |
| Number of pages | 5 |
| Journal | International Immunology |
| Volume | 3 |
| Issue number | 8 |
| DOIs | |
| State | Published - Aug 1991 |
Bibliographical note
Funding Information:This research was supported by the Wolfson Research Award administered by the Israel Academy of Science and Humanities, the research associates of the Lautenberg Center, and the Concern Foundation of Los Angeles. We thank Dr Gary Nabel for the recombinant IL-3, Dr Yehiel Zick for providing B8 and 810 human autoimmune sera, and Drs Efrat Wertheimer and Shlomo Sasson for their advice in designing the uptake assays.
Keywords
- Hexose transport
- IL-3 response
- Lymphocyte activation