Analysis of multicellular patterns is required to understand tissue organizational processes. By using a multi-scale object oriented image processing method, the spatial information of cells can be extracted automatically. Instead of manual segmentation or indirect measurements, such as general distribution of contrast or flow, the orientation and distribution of individual cells is extracted for quantitative analysis. Relevant objects are identified by feature queries and no low-level knowledge of image processing is required.
Bibliographical notePublisher Copyright:
© 2016 Christian Thies et al., licensee De Gruyter.
- Cell segmentation
- Image analysis
- Object extraction
- Phase contrast micrographs
- Spatial information
- Tissue organization