Imaging techniques for assaying lymphocyte activation in action

Lakshmi Balagopalan; Eilon Sherman; Valarie A. Barr; Lawrence E. Samelson

doi:10.1038/nri2903

Imaging techniques for assaying lymphocyte activation in action

Lakshmi Balagopalan, Eilon Sherman, Valarie A. Barr, Lawrence E. Samelson

Research output: Contribution to journal › Article › peer-review

74 Scopus citations

Abstract

Imaging techniques have greatly improved our understanding of lymphocyte activation. Technical advances in spatial and temporal resolution and new labelling tools have enabled researchers to directly observe the activation process. Consequently, research using imaging approaches to study lymphocyte activation has expanded, providing an unprecedented level of cellular and molecular detail in the field. As a result, certain models of lymphocyte activation have been verified, others have been revised and yet others have been replaced with new concepts. In this article, we review the current imaging techniques that are used to assess lymphocyte activation in different contexts, from whole animals to single molecules, and discuss the advantages and potential limitations of these methods.

Original language	American English
Pages (from-to)	21-33
Number of pages	13
Journal	Nature Reviews Immunology
Volume	11
Issue number	1
DOIs	https://doi.org/10.1038/nri2903
State	Published - Jan 2011
Externally published	Yes

Bibliographical note

Funding Information:
We thank R. Kortum for critically reading the manuscript. This research was supported by the Intramural Research Program of the National Institutes of Health (NIH), National Cancer Institute (NCI), Center for Cancer Research (CCR).

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abstract = "Imaging techniques have greatly improved our understanding of lymphocyte activation. Technical advances in spatial and temporal resolution and new labelling tools have enabled researchers to directly observe the activation process. Consequently, research using imaging approaches to study lymphocyte activation has expanded, providing an unprecedented level of cellular and molecular detail in the field. As a result, certain models of lymphocyte activation have been verified, others have been revised and yet others have been replaced with new concepts. In this article, we review the current imaging techniques that are used to assess lymphocyte activation in different contexts, from whole animals to single molecules, and discuss the advantages and potential limitations of these methods.",

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note = "Funding Information: We thank R. Kortum for critically reading the manuscript. This research was supported by the Intramural Research Program of the National Institutes of Health (NIH), National Cancer Institute (NCI), Center for Cancer Research (CCR).",

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AU - Barr, Valarie A.

AU - Samelson, Lawrence E.

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AB - Imaging techniques have greatly improved our understanding of lymphocyte activation. Technical advances in spatial and temporal resolution and new labelling tools have enabled researchers to directly observe the activation process. Consequently, research using imaging approaches to study lymphocyte activation has expanded, providing an unprecedented level of cellular and molecular detail in the field. As a result, certain models of lymphocyte activation have been verified, others have been revised and yet others have been replaced with new concepts. In this article, we review the current imaging techniques that are used to assess lymphocyte activation in different contexts, from whole animals to single molecules, and discuss the advantages and potential limitations of these methods.

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Imaging techniques for assaying lymphocyte activation in action

Abstract

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