IME1, a positive regulator gene of meiosis in S. cerevisiae

Yona Kassir; David Granot; Giora Simchen

doi:10.1016/0092-8674(88)90427-8

IME1, a positive regulator gene of meiosis in S. cerevisiae

Yona Kassir^*, David Granot, Giora Simchen

^*Corresponding author for this work

Alexander Silberman Institute of Life Sciences

Research output: Contribution to journal › Article › peer-review

247 Scopus citations

Abstract

IMEI (inducer of MEiosis) was cloned due to its high copy number effect: it enabled MAT insufficient strains to undergo meiosis. Disruption of IMEI results in a recessive Spo^- phenotype. Diploids homozygous for the two mutations ime1-0, rmel-1 are also meios deficient. We conclude that IME1 is a positive regultor of meiosis that normally is repressed by RME1. RME is repressed by a complex of MATal and MATα2 gene products. IME1 is also regulated by the environment: no transcripts could be detected in glucose growing cells, in contrast to acetate growing cell. Starvation for nitrogen further induced (6- to 8-fold) transcription of IME1, but, as expected, the induction was found only in MATa/MATα or rmel-l/rme1-1 diploids. Furthermore, the IME1 multicopy plasmids promoted sporulation in rich media.

Original language	English
Pages (from-to)	853-862
Number of pages	10
Journal	Cell
Volume	52
Issue number	6
DOIs	https://doi.org/10.1016/0092-8674(88)90427-8
State	Published - 25 Mar 1988

Access to Document

10.1016/0092-8674(88)90427-8

Cite this

@article{662a4945495c4c90a6e84fd24ef8bc78,

title = "IME1, a positive regulator gene of meiosis in S. cerevisiae",

abstract = "IMEI (inducer of MEiosis) was cloned due to its high copy number effect: it enabled MAT insufficient strains to undergo meiosis. Disruption of IMEI results in a recessive Spo- phenotype. Diploids homozygous for the two mutations ime1-0, rmel-1 are also meios deficient. We conclude that IME1 is a positive regultor of meiosis that normally is repressed by RME1. RME is repressed by a complex of MATal and MATα2 gene products. IME1 is also regulated by the environment: no transcripts could be detected in glucose growing cells, in contrast to acetate growing cell. Starvation for nitrogen further induced (6- to 8-fold) transcription of IME1, but, as expected, the induction was found only in MATa/MATα or rmel-l/rme1-1 diploids. Furthermore, the IME1 multicopy plasmids promoted sporulation in rich media.",

author = "Yona Kassir and David Granot and Giora Simchen",

year = "1988",

month = mar,

day = "25",

doi = "10.1016/0092-8674(88)90427-8",

language = "אנגלית",

volume = "52",

pages = "853--862",

journal = "Cell",

issn = "0092-8674",

publisher = "Elsevier B.V.",

number = "6",

}

TY - JOUR

T1 - IME1, a positive regulator gene of meiosis in S. cerevisiae

AU - Kassir, Yona

AU - Granot, David

AU - Simchen, Giora

PY - 1988/3/25

Y1 - 1988/3/25

N2 - IMEI (inducer of MEiosis) was cloned due to its high copy number effect: it enabled MAT insufficient strains to undergo meiosis. Disruption of IMEI results in a recessive Spo- phenotype. Diploids homozygous for the two mutations ime1-0, rmel-1 are also meios deficient. We conclude that IME1 is a positive regultor of meiosis that normally is repressed by RME1. RME is repressed by a complex of MATal and MATα2 gene products. IME1 is also regulated by the environment: no transcripts could be detected in glucose growing cells, in contrast to acetate growing cell. Starvation for nitrogen further induced (6- to 8-fold) transcription of IME1, but, as expected, the induction was found only in MATa/MATα or rmel-l/rme1-1 diploids. Furthermore, the IME1 multicopy plasmids promoted sporulation in rich media.

AB - IMEI (inducer of MEiosis) was cloned due to its high copy number effect: it enabled MAT insufficient strains to undergo meiosis. Disruption of IMEI results in a recessive Spo- phenotype. Diploids homozygous for the two mutations ime1-0, rmel-1 are also meios deficient. We conclude that IME1 is a positive regultor of meiosis that normally is repressed by RME1. RME is repressed by a complex of MATal and MATα2 gene products. IME1 is also regulated by the environment: no transcripts could be detected in glucose growing cells, in contrast to acetate growing cell. Starvation for nitrogen further induced (6- to 8-fold) transcription of IME1, but, as expected, the induction was found only in MATa/MATα or rmel-l/rme1-1 diploids. Furthermore, the IME1 multicopy plasmids promoted sporulation in rich media.

UR - http://www.scopus.com/inward/record.url?scp=0024296512&partnerID=8YFLogxK

U2 - 10.1016/0092-8674(88)90427-8

DO - 10.1016/0092-8674(88)90427-8

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 3280136

AN - SCOPUS:0024296512

SN - 0092-8674

VL - 52

SP - 853

EP - 862

JO - Cell

JF - Cell

IS - 6

ER -

IME1, a positive regulator gene of meiosis in S. cerevisiae

Abstract

Access to Document

Other files and links

Fingerprint

Cite this