Abstract
The cytotoxic immune response of (A X C57BL/6)F1 hybrids to the in vivo carried (YAC) and in vitro carried (YAC-1) Moloney-induced tumors was determined and compared with the anti-YAC or YAC-1 syngeneic cytotoxic responses of splenocytes from the A parent strain. In addition, the cytotoxic response of (A X C57BL/6)F1 hybrids to a Rauscher-induced tumor RBL5 was determined and compared with the anti-RBL5 syngeneic cytotoxic response of splenocytes from C57BL/6 mice. It was found that the in vitro anti-YAC or YAC-1 cytotoxic responses of the hybrid spleen cells were completely dependent on the immunizing of the donors of the cells with the relevant tumors. In contrast, the anti-RBL5 cytotoxic response of the hybrid splenocytes was generated after in vitro sensitization with RBL5, even in cultures of nonprimed spleen cells. Cultures of non-primed C57BL/6 splenocytes did not generate a RBL5 cytotoxic response in similar conditions. This phenomenon was discussed in terms of the 'hybrid responsiveness' effect. It was concluded that the immune response of the hybrids nearly duplicated the syngeneic immune response of the A parent strain and differed from the syngeneic immune response of the C57BL/6 parent strain. YAC-1 carried in vivo induced the appearance of suppressor cells in the spleens of (A X C57BL/6)F1 mice. These suppressor cells inhibited the enhanced cytotoxic response of RBL5-primed splenocytes of hybrid mice after co-cultivation with RBL5 tumor. However, the hybrid suppressor cells did not inhibit the enhanced anti-RBL5 cytotoxic response of YAC-1 primed splenocytes nor did they inhibit the anti-YAC or YAC-1 cytotoxic responses of YAC-1 or RBL5-primed splenocytes.
Original language | English |
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Pages (from-to) | 988-994 |
Number of pages | 7 |
Journal | Journal of Immunology |
Volume | 125 |
Issue number | 3 |
State | Published - 1980 |