Immunization of mice with syngeneic moloney lymphoma cells induces separate antibodies against virion envelope glycoprotein and virus-induced cell surface antigens*

E. M. Fenyö, E. Yefenof, E. Klein, G. Klein

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Immunization of mice with heavily irradiated syngeneic Moloney lymphoma cells evokes antibodies against the major viral envelope antigen, gp71, and the Moloney virus-induced cell surface antigen (MCSA). A9HT cells, an L-cell subline, react with the antibodies against the viral envelope antigen only; this reaction can be completely inhibited by virus or purified gp71. Reactivity to Moloney lymphoma cells (YAC) was only partially inhibited (maximum 30%) or not at all. This can be attributed to the reaction of the YAC cells with antibodies directed against MCSA, a nonvirion cell surface component according to both biological and biochemical evidence. Antibody-induced capping of gp71 or plS(E) did not change the membrane distribution of MCSA or H-2, indicating that these antigens represent distinct entities on the cell surface. MCSA showed only minimal capping and thereby differed in behavior from both H-2 and virion antigens, gp71 could be capped by the mouse antiserum as revealed by subsequent staining with monospecific anti-gp71 antiserum. Under ordinary test conditions this reactivity is overshadowed by the reaction against MCSA. The lack of MCSA capping reflects a difference in anchorage of this antigen.

Original languageEnglish
Pages (from-to)1521-1533
Number of pages13
JournalJournal of Experimental Medicine
Volume146
Issue number6
DOIs
StatePublished - 1 Dec 1977
Externally publishedYes

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