Immunohistochemical analysis of the segregation process of the quail germ cell lineage

M. Ginsburg, J. Hochman, H. Eyal-Giladi

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

An antiserum against quail 7 day gonadal germ cells was found to react specifically with gonadal germ cells of both sexes. Transverse sections from a range of early quail developmental stages were submitted to the antibody PAP reaction. Blastodiscs from the earliest uterine stages (II to X E.G and K) reacted very strongly, while the overall reaction gradually decreased in older blastoderms. At stage XIII both epiblast and hypoblast were weakly stained, but some large, PGC-like cells stained intensively. During gastrulation (PS formation) the reaction of the epiblast disppears quicker than that of the hypoblast. The newly formed mesoderm and entoderm do not react at all and the reaction gradually becomes limited mainly to the PGCs and somewhat to the primary hypoblast which is moving into the germinal crescent. The widely spread reaction at the early stages is thus gradually being restricted to the PGCs.

Original languageEnglish
Pages (from-to)389-395
Number of pages7
JournalInternational Journal of Developmental Biology
Volume33
Issue number3
StatePublished - 1989

Fingerprint

Dive into the research topics of 'Immunohistochemical analysis of the segregation process of the quail germ cell lineage'. Together they form a unique fingerprint.

Cite this