Immunohistochemical studies with antibodies to myosins from the cytoplasm and membrane fraction of human blood platelets

Ute Gröschel-Stewart; Christine Rakousky; Renate Franke; Itzhak Peleg; Itkhak Kahane; Amiram Eldor; Andras Muhlrad

doi:10.1007/BF00217186

Immunohistochemical studies with antibodies to myosins from the cytoplasm and membrane fraction of human blood platelets

Ute Gröschel-Stewart^*
, Christine Rakousky
, Renate Franke
, Itzhak Peleg
, Itkhak Kahane
, Amiram Eldor
, Andras Muhlrad

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Antibodies were raised to myosins extracted from the cytoplasm and solubilized membranes of human blood platelets. Both antibodies had similar titers as shown by enzyme-immunoassay and bound to the same sites as shown by immunohistochemistry. They were specific for cytoplasmic myosins (e.g., in human white blood cells, platelets and fibroblasts and rat endothelial cells). They showed no crossreaction with human or rat smooth muscle.

Original language	English
Pages (from-to)	399-404
Number of pages	6
Journal	Cell and Tissue Research
Volume	241
Issue number	2
DOIs	https://doi.org/10.1007/BF00217186
State	Published - Aug 1985
Externally published	Yes

Keywords

Enzyme immunoassay
Immunofluorescence
Man
Myosin
Platelet cytoplasm
Platelet membrane

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10.1007/BF00217186

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title = "Immunohistochemical studies with antibodies to myosins from the cytoplasm and membrane fraction of human blood platelets",

abstract = "Antibodies were raised to myosins extracted from the cytoplasm and solubilized membranes of human blood platelets. Both antibodies had similar titers as shown by enzyme-immunoassay and bound to the same sites as shown by immunohistochemistry. They were specific for cytoplasmic myosins (e.g., in human white blood cells, platelets and fibroblasts and rat endothelial cells). They showed no crossreaction with human or rat smooth muscle.",

keywords = "Enzyme immunoassay, Immunofluorescence, Man, Myosin, Platelet cytoplasm, Platelet membrane",

author = "Ute Gr{\"o}schel-Stewart and Christine Rakousky and Renate Franke and Itzhak Peleg and Itkhak Kahane and Amiram Eldor and Andras Muhlrad",

year = "1985",

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AU - Gröschel-Stewart, Ute

AU - Rakousky, Christine

AU - Franke, Renate

AU - Peleg, Itzhak

AU - Kahane, Itkhak

AU - Eldor, Amiram

AU - Muhlrad, Andras

PY - 1985/8

Y1 - 1985/8

N2 - Antibodies were raised to myosins extracted from the cytoplasm and solubilized membranes of human blood platelets. Both antibodies had similar titers as shown by enzyme-immunoassay and bound to the same sites as shown by immunohistochemistry. They were specific for cytoplasmic myosins (e.g., in human white blood cells, platelets and fibroblasts and rat endothelial cells). They showed no crossreaction with human or rat smooth muscle.

AB - Antibodies were raised to myosins extracted from the cytoplasm and solubilized membranes of human blood platelets. Both antibodies had similar titers as shown by enzyme-immunoassay and bound to the same sites as shown by immunohistochemistry. They were specific for cytoplasmic myosins (e.g., in human white blood cells, platelets and fibroblasts and rat endothelial cells). They showed no crossreaction with human or rat smooth muscle.

KW - Enzyme immunoassay

KW - Immunofluorescence

KW - Man

KW - Myosin

KW - Platelet cytoplasm

KW - Platelet membrane

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ER -

Immunohistochemical studies with antibodies to myosins from the cytoplasm and membrane fraction of human blood platelets

Abstract

Keywords

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