Immunological Cross‐Reactivity between Electric‐Eel Acetylcholinesterase and Rat‐Tail‐Tendon Collagen

Lili ANGLISTER*, Rebecca TARRAB‐HAZDAI, Sara FUCHS, Israel SILMAN

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Immunological cross‐reactivity between acetylcholinesterase from the electric organ of the electric eel and rat tail tendon collagen was examined both on the cellular and humoral levels. Guinea pigs immunized with rat tail tendon collagen displayed a strong delayed‐type skin reaction when tested with the elongated acetylcholinesterase preparation (i.e. 14‐S + 18‐S molecular forms). However, when the glubular 11‐S enzyme was tested, almost no cross‐reactivity was obtained. Similarly, guinea pigs immunized with 14‐S + 18‐S preparation exhibited skin sensitization to rat tail tendon collagen. Using a radioimmunoassay, it was observed that 125I‐labeled 14‐S + 18‐S acetylcholinesterase binds efficiently to rabbit antiserum elicited against rat tail tendon collagen, whereas 125I‐labeled 11‐S enzyme does not bind at all to this antiserum. Similar results were obtained by passive hemagglutination assay. The experiments suggest that 14‐S + 18‐S acetylcholinesterase, but not 11‐S enzyme, which is devoid of the tail structure, has antigenic determinants in common with collagen from rat tail tendon.

Original languageAmerican English
Pages (from-to)25-29
Number of pages5
JournalEuropean Journal of Biochemistry
Volume94
Issue number1
DOIs
StatePublished - Feb 1979
Externally publishedYes

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