TY - JOUR
T1 - Immunomagnetic separation and analysis of non‐malignant variants and parental malignant mouse lymphoma cells
AU - Lazarovici, Philip
AU - Bergel, Michael
AU - Hasson, Tsury
AU - Rahamim, Ezra
AU - Hochman, Jacob
PY - 1991
Y1 - 1991
N2 - We have devised conditions whereby non‐tumorigenic, immunogenic cell variants of S49 mouse lymphoma were analyzed and separated from parental tumorigenic lymphoma cells. This was carried out using polyclonal antibodies (raised against the immunogenic variants) and immunomagnetic beads. The efficacy of the procedure depended on the amount of polyclonal antiserum, the immunobead to cell ratio, incubation time and the number of repetitions of the procedure. Experiments with mixed tumorigenic and non‐tumorigenic cells have resulted in an enrichment of up to 200‐fold of the non‐tumorigenic, immunogenic cells in the population. These findings indicate the potential use of this procedure (in conjunction with other approaches) to isolate from a population of tumorigenic cells those variant cells that might be used to immunize against the parental tumor.
AB - We have devised conditions whereby non‐tumorigenic, immunogenic cell variants of S49 mouse lymphoma were analyzed and separated from parental tumorigenic lymphoma cells. This was carried out using polyclonal antibodies (raised against the immunogenic variants) and immunomagnetic beads. The efficacy of the procedure depended on the amount of polyclonal antiserum, the immunobead to cell ratio, incubation time and the number of repetitions of the procedure. Experiments with mixed tumorigenic and non‐tumorigenic cells have resulted in an enrichment of up to 200‐fold of the non‐tumorigenic, immunogenic cells in the population. These findings indicate the potential use of this procedure (in conjunction with other approaches) to isolate from a population of tumorigenic cells those variant cells that might be used to immunize against the parental tumor.
UR - http://www.scopus.com/inward/record.url?scp=0026185478&partnerID=8YFLogxK
U2 - 10.1002/jmr.300040406
DO - 10.1002/jmr.300040406
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C2 - 1799463
AN - SCOPUS:0026185478
SN - 0952-3499
VL - 4
SP - 143
EP - 149
JO - Journal of Molecular Recognition
JF - Journal of Molecular Recognition
IS - 4
ER -