Impaired purine biosynthesis affects pathogenicity of Fusarium oxysporum f. sp. melonis

Youlia Denisov, Oded Yarden, Stanley Freeman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


The vascular wilt pathogen Fusarium oxysporum f. sp. melonis causes worldwide yield losses of muskmelon. In this study, we characterized a UV-induced non-pathogenic mutant (strain 4/4) of F. oxysporum f. sp. melonis, previously identified as a potential biological control agent. During comparative analysis of vegetative growth parameters using different carbon sources, mutant strain 4/4 showed a delay in development and secretion of extracellular enzymes, compared to the wild type strain. Amendments of the growth medium with yeast extract, adenine or hypoxanthine, but not guanine, complemented the growth defect of strain 4/4, as well as secretion and partial activity of cellulases and endopolygalacturonases, indicating that the strain is an adenine auxotroph. Incubation of strain 4/4 conidia in adenine solution, prior to inoculation of muskmelon plants, partially restored pathogenicity to the mutant strain.

Original languageAmerican English
Pages (from-to)293-297
Number of pages5
JournalEuropean Journal of Plant Pathology
Issue number3
StatePublished - Jul 2005

Bibliographical note

Funding Information:
We would like to thank Zeraim Gedera, Israel, for providing us with muskmelon seeds. This study was partially supported by Grant 132-0922 from the Chief Scientist of the Israeli Ministry of Agriculture awarded to S. Freeman. Contribution No. 515/04 from the Institute of Plant Protection, ARO, Volcani Center, Bet Dagan, Israel.


  • Fusarium wilt
  • Purine auxotroph
  • UV-induced mutant


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