Impedimetric or ion-sensitive field-effect transistor (ISFET) aptasensors based on the self-assembly of au nanoparticle-functionalized supramolecular aptamer nanostructures

Etery Sharon; Ronit Freeman; Tel Vered Ran; Itamar Willner

doi:10.1002/elan.200804565

Impedimetric or ion-sensitive field-effect transistor (ISFET) aptasensors based on the self-assembly of au nanoparticle-functionalized supramolecular aptamer nanostructures

Etery Sharon, Ronit Freeman, Tel Vered Ran, Itamar Willner^*

^*Corresponding author for this work

Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

Two electrical methods (Faradaic impedance spectroscopy and ion-sensitive field-effect transistor (ISFET)) are applied to sense aptamer-substrate complexes. The methods utilize anticocaine aptamer fragments that self-assemble, in the presence of cocaine, to a supramolecular aptamer fragments/cocaine complex on the electrode surface or ISFET gate. One of the aptamer fragments is assembled on a Au electrode or the ISFET gate. The second thiolated aptamer fragment is used to modify Au NPs that are used as amplifying labels for the two detection schemes. The impedimetric and ISFET methods enabled the analysis of cocaine with detection limits of 1×10^-5 M and 1×10 ^-6 M, respectively.

Original language	English
Pages (from-to)	1291-1296
Number of pages	6
Journal	Electroanalysis
Volume	21
Issue number	11
DOIs	https://doi.org/10.1002/elan.200804565
State	Published - Jun 2009

Keywords

Aptamers
Cocaine
Field-effect-Transistors
Impedance
Sensors

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10.1002/elan.200804565

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abstract = "Two electrical methods (Faradaic impedance spectroscopy and ion-sensitive field-effect transistor (ISFET)) are applied to sense aptamer-substrate complexes. The methods utilize anticocaine aptamer fragments that self-assemble, in the presence of cocaine, to a supramolecular aptamer fragments/cocaine complex on the electrode surface or ISFET gate. One of the aptamer fragments is assembled on a Au electrode or the ISFET gate. The second thiolated aptamer fragment is used to modify Au NPs that are used as amplifying labels for the two detection schemes. The impedimetric and ISFET methods enabled the analysis of cocaine with detection limits of 1×10-5 M and 1×10 -6 M, respectively.",

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AU - Willner, Itamar

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Impedimetric or ion-sensitive field-effect transistor (ISFET) aptasensors based on the self-assembly of au nanoparticle-functionalized supramolecular aptamer nanostructures

Abstract

Keywords

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