Improved biocontrol activity of Trichoderma harzianum by over-expression of the proteinase encoding gene prb1

Alberto Flores; Ilan Chet; Alfredo Herrera-Estrella

doi:10.1007/s002940050173

Improved biocontrol activity of Trichoderma harzianum by over-expression of the proteinase encoding gene prb1

Alberto Flores, Ilan Chet, Alfredo Herrera-Estrella^*

^*Corresponding author for this work

Department of Plant Pathology and Microbiology

Research output: Contribution to journal › Article › peer-review

158 Scopus citations

Abstract

Transformation systems developed for Trichoderma spp. were utilized to improve the biocontrol efficiency of the mycoparasitic fungus Trichoderma harzianum by increasing the copy number of the basic proteinase gene prb1. The transformants were stable and carried from two to ten copies of prb1. High levels of expression of prb1 during fungus-fungus interaction were detected when T. harzianum and Rhizoctonia solani were confronted in vitro. In liquid cultures the proteinase was induced by cell walls of R. solani. Under greenhouse conditions, incorporation of T. harzianum transformants into pathogen-infested soil significantly reduced the disease caused by R. solani in cotton plants.

Original language	English
Pages (from-to)	30-37
Number of pages	8
Journal	Current Genetics
Volume	31
Issue number	1
DOIs	https://doi.org/10.1007/s002940050173
State	Published - Jan 1997

Keywords

Biocontrol
Mycoparasitism
Proteinase
Transformation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s002940050173

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abstract = "Transformation systems developed for Trichoderma spp. were utilized to improve the biocontrol efficiency of the mycoparasitic fungus Trichoderma harzianum by increasing the copy number of the basic proteinase gene prb1. The transformants were stable and carried from two to ten copies of prb1. High levels of expression of prb1 during fungus-fungus interaction were detected when T. harzianum and Rhizoctonia solani were confronted in vitro. In liquid cultures the proteinase was induced by cell walls of R. solani. Under greenhouse conditions, incorporation of T. harzianum transformants into pathogen-infested soil significantly reduced the disease caused by R. solani in cotton plants.",

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Improved biocontrol activity of Trichoderma harzianum by over-expression of the proteinase encoding gene prb1

Abstract

Keywords

UN SDGs

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