Improved single-step PCR assay for sex identification post-allogeneic sex-mismatched BMT

T. Pugatsch*, A. Oppenheim, S. Slavin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

56 Scopus citations

Abstract

A method is presented which allows sex-identification simultaneously of both male and female cells in cell mixtures. The sensitivity of this PCR-based assay is one male cell in 10 6 female cells. The test can be used routinely to analyze bone marrow and peripheral blood lymphocytes post-allogeneic sex-mismatched bone marrow transplantation to detect early engraftment or rejection, as well as host cell regeneration.

Original languageEnglish
Pages (from-to)273-275
Number of pages3
JournalBone Marrow Transplantation
Volume17
Issue number2
StatePublished - Feb 1996
Externally publishedYes

Keywords

  • BMT
  • PCR
  • Sex identification

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