Improved single-step PCR assay for sex identification post-allogeneic sex-mismatched BMT

T. Pugatsch; A. Oppenheim; S. Slavin

Improved single-step PCR assay for sex identification post-allogeneic sex-mismatched BMT

T. Pugatsch^*
, A. Oppenheim
, S. Slavin

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

A method is presented which allows sex-identification simultaneously of both male and female cells in cell mixtures. The sensitivity of this PCR-based assay is one male cell in 10 ⁶ female cells. The test can be used routinely to analyze bone marrow and peripheral blood lymphocytes post-allogeneic sex-mismatched bone marrow transplantation to detect early engraftment or rejection, as well as host cell regeneration.

Original language	English
Pages (from-to)	273-275
Number of pages	3
Journal	Bone Marrow Transplantation
Volume	17
Issue number	2
State	Published - Feb 1996
Externally published	Yes

Keywords

BMT
PCR
Sex identification

Cite this

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title = "Improved single-step PCR assay for sex identification post-allogeneic sex-mismatched BMT",

abstract = "A method is presented which allows sex-identification simultaneously of both male and female cells in cell mixtures. The sensitivity of this PCR-based assay is one male cell in 10 6 female cells. The test can be used routinely to analyze bone marrow and peripheral blood lymphocytes post-allogeneic sex-mismatched bone marrow transplantation to detect early engraftment or rejection, as well as host cell regeneration.",

keywords = "BMT, PCR, Sex identification",

author = "T. Pugatsch and A. Oppenheim and S. Slavin",

year = "1996",

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N2 - A method is presented which allows sex-identification simultaneously of both male and female cells in cell mixtures. The sensitivity of this PCR-based assay is one male cell in 10 6 female cells. The test can be used routinely to analyze bone marrow and peripheral blood lymphocytes post-allogeneic sex-mismatched bone marrow transplantation to detect early engraftment or rejection, as well as host cell regeneration.

AB - A method is presented which allows sex-identification simultaneously of both male and female cells in cell mixtures. The sensitivity of this PCR-based assay is one male cell in 10 6 female cells. The test can be used routinely to analyze bone marrow and peripheral blood lymphocytes post-allogeneic sex-mismatched bone marrow transplantation to detect early engraftment or rejection, as well as host cell regeneration.

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KW - PCR

KW - Sex identification

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Improved single-step PCR assay for sex identification post-allogeneic sex-mismatched BMT

Abstract

Keywords

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