Improvement of sperm cryo-survival of cynomolgus macaque (Macaca fascicularis) by commercial egg-yolk–free freezing medium with type III antifreeze protein

Shengnan Wang, Yanchao Duan, Yaping Yan, Chen Adar, Ido Braslavsky, Bingbing Chen, Tianzhuang Huang, Shuai Qiu, Xi Li, Briauna Marie Inglis, Weizhi Ji, Wei Si*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


When nonhuman primate sperm undergoes cryopreservation in an egg yolk medium there is an increased risk that the egg yolk might adversely affect the sperm due to containing of avian pathogens. Although commercial egg-yolk-free medium for human sperm cryopreservation has been used for macaque sperm, the cryo-survival remains less than optimal. The present study, therefore, was conducted to determine the optimal concentration of antifreeze protein (AFP) III supplemented in a commercial egg-yolk-free medium for cynomolgus macaque (Macaca fascicularis) sperm cryo-survival. The function of frozen-thawed sperm was evaluated by post-thaw sperm motility, acrosome integrity, and mitochondrial function. Results indicate that the sperm motilities were greater when 0.1, 1, and 10 μg/ml of AFP III were supplemented into the sperm freezing medium (P < 0.05). In addition, the mitochondrial membrane potential was greater in the sperm cryopreserved with the medium that was supplemented with 0.1 μg/ml of AFP III (P < 0.05). The addition of AFP III at any of the concentrations, however, did not have any cryoprotection effect on the sperm acrosome, and the greatest concentrations of AFP III at 100 and 200 μg/ml had detrimental effects on acrosomal integrity (P < 0.05). Results of the present study indicated the methods used are effective for the cryopreservation of cynomolgus monkey sperm while reducing associated health risks due to avian pathogens being present in egg yolk-based extenders.

Original languageAmerican English
Article number106177
JournalAnimal Reproduction Science
StatePublished - Nov 2019

Bibliographical note

Funding Information:
This research was supported by grants from the National Natural Science Foundation of China [grant number: 31660346 ] and the National Key Research and Development Program of China [grant number: 2016YFA0101400 ].

Publisher Copyright:
© 2019 Elsevier B.V.


  • Antifreeze protein
  • Cynomolgus macaque
  • Egg-yolk-free
  • Sperm cryopreservation


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