Impurities of [1-13C]Pyruvic Acid and a Method to Minimize Their Signals for Hyperpolarized Pyruvate Metabolism Studies

Talia Harris; Ayelet Gamliel; Jacob Sosna; J. Moshe Gomori; Rachel Katz-Brull

doi:10.1007/s00723-018-1030-1

Impurities of [1-¹³C]Pyruvic Acid and a Method to Minimize Their Signals for Hyperpolarized Pyruvate Metabolism Studies

Talia Harris, Ayelet Gamliel, Jacob Sosna, J. Moshe Gomori, Rachel Katz-Brull^*

^*Corresponding author for this work

Faculty of Medicine

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Impurities are a necessary evil when working with hyperpolarized [1-¹³C]pyruvate for real-time metabolic studies. We show that the impurities’ spectrum persists throughout the detection window as these signals show a long T₁. At the same time, the impurities’ spectrum is unpredictable and partly susceptible to minute pH changes in the microenvironment such that impurities’ signals may overlap with hyperpolarized metabolite signals. We show a method to eliminate the hyperpolarized impurities’ signals while maintaining sufficient hyperpolarized [1-¹³C]pyruvate signal for metabolic studies.

Original language	English
Pages (from-to)	1085-1098
Number of pages	14
Journal	Applied Magnetic Resonance
Volume	49
Issue number	10
DOIs	https://doi.org/10.1007/s00723-018-1030-1
State	Published - 1 Oct 2018

Bibliographical note

Funding Information:
Funding Sponsor: This project has received funding from the European Research Council (ERC) under grant agreement No. 338040 to R.K-B) and from the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 667192.

Publisher Copyright:
© 2018, Springer-Verlag GmbH Austria, part of Springer Nature.

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Impurities of [1-¹³C]Pyruvic Acid and a Method to Minimize Their Signals for Hyperpolarized Pyruvate Metabolism Studies

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