In situ SFM study of 2D-polyaniline surface-confined enzymatic polymerization

Ruthy Sfez, Naama Peor, Sidney R. Cohen, Hagai Cohen, Shlomo Yitzchaik*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


In this contribution we report on an in situ enzymatic self-assembly of a polyaniline (PAN) monolayer on modified hydroxyl-terminated surfaces. The consecutive assembly steps consist of a chemical deposition of 3-aminopropyltrimethoxysilane (APT) as a coupling agent with a positively-charged amine end group. The next step involved an electrostatic adhesion of sulfonated polystyrene (SPS) followed by electrostatic adhesion of anilinium. In situ enzymatic polymerization of the anilinium monolayer took place using horseradish peroxidase (HRP) enzyme and its substrate H 2O2. The assembly steps were characterized by variable angle spectroscopic ellipsometry (VASE), UV-Vis-NIR spectroscopy, contact angle measurements, X-ray photoelectron spectroscopy (XPS), contact potential difference (CPD) and scanning force microscopy (SFM). The SFM measurements were divided between ex situ analysis in which morphology of the obtained layers was determined, and in situ analysis which provided information on the dynamic process of the enzymatic polymerization.

Original languageAmerican English
Pages (from-to)4044-4050
Number of pages7
JournalJournal of Materials Chemistry
Issue number41
StatePublished - 2006


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