This paper describes a low-cost system for in situ visualization and photoablation of single neurons. The system includes a fiber optics light source equipped with a filter port, a blue excitation filter and a yellow barrier filter, and a light guide which terminates with a focusing lens. This system is inexpensive, easy to use, and requires minimal maintenance. Given its price, this system is readily accessible and has the potential of becoming standard equipment for in situ visualization and killing of individual neurons.
Bibliographical noteFunding Information:
We thank J.M. Camhi, Z. Silverman and A. Weisel Eichler for editing and critically reading the manuscript. This research was supported by grant No 93-00048 from the United States-Israel Binational Science Foundation (BSF), Jerusalem, Israel.
- Fiber optic
- Fluorescent dye
- Identified neuron
- In situ visualization
- Neuronal circuitry