In-vitro and in-vivo biological activity of recombinant yellowtail kingfish (Seriola lalandi) follicle stimulating hormone

Pablo J. Sanchís-Benlloch; Josephine Nocillado; Claudia Ladisa; Joseph Aizen; Adam Miller; Michal Shpilman; Berta Levavi-Sivan; Tomer Ventura; Abigail Elizur

doi:10.1016/j.ygcen.2016.03.001

In-vitro and in-vivo biological activity of recombinant yellowtail kingfish (Seriola lalandi) follicle stimulating hormone

Pablo J. Sanchís-Benlloch, Josephine Nocillado, Claudia Ladisa, Joseph Aizen, Adam Miller, Michal Shpilman, Berta Levavi-Sivan, Tomer Ventura, Abigail Elizur^*

^*Corresponding author for this work

Department of Animal Sciences

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

Biologically active recombinant yellowtail kingfish follicle stimulating hormone (rytkFsh) was produced in yeast Pichia pastoris and its biological activity was demonstrated by both in-vitro and in-vivo bioassays. Incubation of ovarian and testicular fragments with the recombinant hormone stimulated E₂ and 11-KT secretion, respectively. In-vivo trial in immature female YTK resulted in a significant increase of plasma E₂ levels and development of oocytes. In males at the early stages of puberty, advancement of spermatogenesis was observed, however plasma 11-KT levels were reduced when administered with rytkFsh.

Original language	English
Pages (from-to)	41-49
Number of pages	9
Journal	General and Comparative Endocrinology
Volume	241
DOIs	https://doi.org/10.1016/j.ygcen.2016.03.001
State	Published - 15 Jan 2017

Bibliographical note

Publisher Copyright:
© 2016 Elsevier Inc.

Keywords

FSH
Pichia pastoris
Recombinant protein
Seriola lalandi
Teleost puberty

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10.1016/j.ygcen.2016.03.001

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abstract = "Biologically active recombinant yellowtail kingfish follicle stimulating hormone (rytkFsh) was produced in yeast Pichia pastoris and its biological activity was demonstrated by both in-vitro and in-vivo bioassays. Incubation of ovarian and testicular fragments with the recombinant hormone stimulated E2 and 11-KT secretion, respectively. In-vivo trial in immature female YTK resulted in a significant increase of plasma E2 levels and development of oocytes. In males at the early stages of puberty, advancement of spermatogenesis was observed, however plasma 11-KT levels were reduced when administered with rytkFsh.",

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AU - Nocillado, Josephine

AU - Ladisa, Claudia

AU - Aizen, Joseph

AU - Miller, Adam

AU - Shpilman, Michal

AU - Levavi-Sivan, Berta

AU - Ventura, Tomer

AU - Elizur, Abigail

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KW - Pichia pastoris

KW - Recombinant protein

KW - Seriola lalandi

KW - Teleost puberty

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In-vitro and in-vivo biological activity of recombinant yellowtail kingfish (Seriola lalandi) follicle stimulating hormone

Abstract

Bibliographical note

Keywords

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