In-vitro and in-vivo biological activity of recombinant yellowtail kingfish (Seriola lalandi) follicle stimulating hormone

Pablo J. Sanchís-Benlloch, Josephine Nocillado, Claudia Ladisa, Joseph Aizen, Adam Miller, Michal Shpilman, Berta Levavi-Sivan, Tomer Ventura, Abigail Elizur*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Biologically active recombinant yellowtail kingfish follicle stimulating hormone (rytkFsh) was produced in yeast Pichia pastoris and its biological activity was demonstrated by both in-vitro and in-vivo bioassays. Incubation of ovarian and testicular fragments with the recombinant hormone stimulated E2 and 11-KT secretion, respectively. In-vivo trial in immature female YTK resulted in a significant increase of plasma E2 levels and development of oocytes. In males at the early stages of puberty, advancement of spermatogenesis was observed, however plasma 11-KT levels were reduced when administered with rytkFsh.

Original languageAmerican English
Pages (from-to)41-49
Number of pages9
JournalGeneral and Comparative Endocrinology
Volume241
DOIs
StatePublished - 15 Jan 2017

Bibliographical note

Publisher Copyright:
© 2016 Elsevier Inc.

Keywords

  • FSH
  • Pichia pastoris
  • Recombinant protein
  • Seriola lalandi
  • Teleost puberty

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