TY - JOUR
T1 - In vitro and in vivo evaluation of carboplatin delivery to the eye using hydrogel-iontophoresis
AU - Eljarrat-Binstock, Esther
AU - Domb, Abraham J.
AU - Orucov, Faik
AU - Dagan, Amir
AU - Frucht-Pery, Joseph
AU - Pe'Er, Jacob
PY - 2008
Y1 - 2008
N2 - Purpose: To investigate in vitro and in vivo hydrogel-iontophoresis delivery of carboplatin to the eye. Methods: Iontophoresis was applied on agar gels resembling the eye using different current intensities and durations. Transscleral iontophoresis was performed on healthy rabbits, applying 0, 1, and 3 mA current for 10 min. Results: Similar drug concentrations were obtained in all experimental groups, in in vitro and in vivo studies, regardless of the iontophoretic current applied. A 20-mm penetration depth was found for carboplatin at the agar model. High drug levels were found at the sclera and retina, while lower levels were found at ocular fluids. Conclusion: Carboplatin-iontophoretic application at the above conditions does not have an obvious advantage over passive penetration due to high diffusion properties and insufficient molecular charge. Passive carboplatin diffusion from loaded hydrogels inserted in the lower cul-de-sac should be further investigated as a potential clinical treatment for intraocular retinoblastoma.
AB - Purpose: To investigate in vitro and in vivo hydrogel-iontophoresis delivery of carboplatin to the eye. Methods: Iontophoresis was applied on agar gels resembling the eye using different current intensities and durations. Transscleral iontophoresis was performed on healthy rabbits, applying 0, 1, and 3 mA current for 10 min. Results: Similar drug concentrations were obtained in all experimental groups, in in vitro and in vivo studies, regardless of the iontophoretic current applied. A 20-mm penetration depth was found for carboplatin at the agar model. High drug levels were found at the sclera and retina, while lower levels were found at ocular fluids. Conclusion: Carboplatin-iontophoretic application at the above conditions does not have an obvious advantage over passive penetration due to high diffusion properties and insufficient molecular charge. Passive carboplatin diffusion from loaded hydrogels inserted in the lower cul-de-sac should be further investigated as a potential clinical treatment for intraocular retinoblastoma.
KW - Carboplatin
KW - Eye
KW - Hydrogel
KW - Iontophoresis
KW - Retinoblastoma
UR - http://www.scopus.com/inward/record.url?scp=44049088856&partnerID=8YFLogxK
U2 - 10.1080/02713680701871140
DO - 10.1080/02713680701871140
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C2 - 18350438
AN - SCOPUS:44049088856
SN - 0271-3683
VL - 33
SP - 269
EP - 275
JO - Current Eye Research
JF - Current Eye Research
IS - 3
ER -