In vitro antimicrobial effect of RC-prep within dentinal tubules

Ilana Heling*, Eldad Irani, Sunil Karni, Doron Steinberg

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


The antimicrobial effect of RC-Prep and its components - 10% urea peroxide, 15% EDTA, and glycol - were evaluated individually, in vitro, within dentinal tubules. Standardized bovine incisor root specimens that had been infected with either Staphylococcus aureus or Streptococcus peltzer were incubated with each agent for 10 and 45 min. After exposure to the agent, dentin samples were collected from within the lumina using ISO 23 to 31 burs. Dentin powder samples were incubated with bacterial broth, and the amount of bacteria present was assessed by spectrophotometry. All agents tested were more effective in killing bacteria after incubation for 45 min than after 10 min.

Original languageAmerican English
Pages (from-to)782-785
Number of pages4
JournalJournal of Endodontics
Issue number12
StatePublished - 1999


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