TY - JOUR
T1 - In vitro binding interactions of oral bacteria with immobilized fructosyltransferase
AU - Shemesh, M.
AU - Steinberg, D.
PY - 2006/4
Y1 - 2006/4
N2 - Aims: The objective of the present study was to explore the role of immobilized fructosyltransferase (FTF) in adhesion process. Methods and Results: We investigated real-time biospecific interactions between several types of oral bacteria and recombinant FTF immobilized on a biosensor chip, using surface plasmon resonance technology. Streptococcus mutans, Streptococcus sobrinus and Actinomyces viscosus demonstrated significant binding to FTF. Actinomyces viscosus had a greater binding to FTF, with 373 Resonance Units (RU), than the other tested bacteria. The binding level to FTF of Strep. sobrinus was 320 RU, whereas Strep. mutans and Streptococcus salivarious show binding of 296 and 245 RU, respectively. The binding sensograms displayed different profiles for the tested bacteria at various cell density, suggesting a different affinity to immobilized FTF. Conclusions: The results from this study suggest that FTF may influence bacterial adherence and colonization of the dental biofilm. Significance and Impact of the Study: The biomolecular interaction analysis enables real-time monitoring of the interaction between adhesions of intact bacteria and their ligands, which might be crucial in the initial phase of biofilm development in vivo.
AB - Aims: The objective of the present study was to explore the role of immobilized fructosyltransferase (FTF) in adhesion process. Methods and Results: We investigated real-time biospecific interactions between several types of oral bacteria and recombinant FTF immobilized on a biosensor chip, using surface plasmon resonance technology. Streptococcus mutans, Streptococcus sobrinus and Actinomyces viscosus demonstrated significant binding to FTF. Actinomyces viscosus had a greater binding to FTF, with 373 Resonance Units (RU), than the other tested bacteria. The binding level to FTF of Strep. sobrinus was 320 RU, whereas Strep. mutans and Streptococcus salivarious show binding of 296 and 245 RU, respectively. The binding sensograms displayed different profiles for the tested bacteria at various cell density, suggesting a different affinity to immobilized FTF. Conclusions: The results from this study suggest that FTF may influence bacterial adherence and colonization of the dental biofilm. Significance and Impact of the Study: The biomolecular interaction analysis enables real-time monitoring of the interaction between adhesions of intact bacteria and their ligands, which might be crucial in the initial phase of biofilm development in vivo.
KW - Bacterial adhesion
KW - Biofilm
KW - Fructosyltransferase
KW - Oral bacteria
KW - SPR
UR - http://www.scopus.com/inward/record.url?scp=33645106907&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2672.2006.02880.x
DO - 10.1111/j.1365-2672.2006.02880.x
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C2 - 16553744
AN - SCOPUS:33645106907
SN - 1364-5072
VL - 100
SP - 871
EP - 877
JO - Journal of Applied Microbiology
JF - Journal of Applied Microbiology
IS - 4
ER -