Abstract
Seasonal growth responses of Fagus sylvatica L. buds to application of hormones, and corresponding changes in proteins, were studied in buds cultured in vitro. Buds were removed from trees at different times during the year and cultured on a basal medium supplemented with indole-3-acetic acid (IAA), gibberellic acid (GA3) and benzylaminopurine (BA). Shoot elongation and leaf expansion of cultured buds were promoted by a combination of IAA, GA3 and BA, and this effect was dependent on the state of bud activity at different seasons. Thus, buds sampled in November elongated slightly in culture and were markedly stimulated by application of growth regulators, but with no leaf expansion. Buds sampled in January and February elongated considerably in vitro even on basal medium, and addition of all 3 hormones promoted both shoot elongation and leaf expansion. GA3 alone was very effective in promoting shoot elongation, but the combination of all 3 hormones was required for extended growth of buds in vitro. Growth modification by hormones was accompanied by changes in the abundance of several polypeptides. An approximately 40 kD polypeptide seems to be specific to GA3 application. The data suggest that the promoting effect of growth regulators on bud growth in vitro is dependent on the degree of innate, seasonal bud dormancy.
Original language | English |
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Pages (from-to) | 136-141 |
Number of pages | 6 |
Journal | Journal of Plant Physiology |
Volume | 138 |
Issue number | 2 |
DOIs | |
State | Published - 1991 |
Keywords
- AC
- aspen culture medium
- BA
- benzylaminopurine
- bud culture
- combination of IAA+GA+BA
- Fagus sylvatica L.
- GA
- gibberellic acid
- growth regulators
- IAA
- IGB
- indole-3-acetic acid
- proteins
- SDS-PAGE
- SDS-PAGE
- sodium dodecyl sulfate polyacrylamide gel electrophoresis